كليدواژه :
Rhus coriaria , استخراج , مشتقات سيليله پروتوكاتكوئيك اسيد , آنتي اكسيدان
چكيده لاتين :
Aims: The aim of this study was to extract, isolate, purify, identify and
determine the percentage of two natural combinations of protocatechoic acid
and gallic acid from Sumac with high efficiency. These compounds exhibit
good antimicrobial, antiviral, antifungal, anti-diabetic, anti-insect and
parasitic and antioxidant properties.
Materials & Methods: In this research, the Rhus coriaria plant from the
trees of the Naghadeh area in East Azarbaijan province, from the trees of the
Aras Baran forests in the East Azarbaijan province and from the trees of the
Shitaneh region in the Kurdistan region of Iraq was collected. To compare
the percentage of their effective ingredients, first in the shadow, they were
dried and ground. In this research, Methanolic and water-methanolic
extractive were first extracted from three sample of Iranian and Iraqi Rhus
coriaria plant by maceration method. Due to the large variety of extractive
compounds from Rhus coriria plant extract, we use column
chromatography, preparative chromatography, thin layer chromatography
and crystallization to identify and purify its two major compounds, Gallic
and Protocatechuic acid, and the two compounds were finally isolated as
white powder. To identify them, we use FT-IR, 1H-NMR, 13C-NMR, GCMass,
DSC and XRD methods. In addition, high-performance liquid
chromatography (HPLC) was used to determine the percentage of gallic and
protouchetic acid as the main antioxidants. Subsequently, to enhance the
lipophilic properties of these compounds, their silyl derivatives were
prepared.
Result and discussion: According to numerous experiments, it can be
concluded that the sample of Iranian Sumac fruits in Arasbaran region has
the highest amounts of gallic acid (2.99%) and protocatechoic acid
(2.902%). We also conclude that the type of soil and the location of growth of a genus are quite influential in its active ingredients, taste and color. By
silylation of these compounds, the lipophilic properties increase sharply,
that is, they pass easily through the cell membrane and increase their
pharmacological effect.
Conclusion: In this work, two natural compounds of high efficiency
protocatechoic acid and gallic acid were extracted, isolated, purified,
identified and their percentage was determined by HPLC. Subsequently,
according to the pharmacological, anti-inflammatory, antibacterial and
antioxidant properties of these compounds, tert-butyldimethylsilyl,
dimethylphenylsilyl, and triethylsilyl derivatives were prepared and their
structure was identified by spectroscopic methods. In this way, the
permeability of the cell membrane is easily done and the pharmacological
effect is greatly increased.
