بررسي فراواني تيلريا اكوئي در اسب‌هاي منطقه ورامين با روش‌هاي رنگ‌آميزي و مولكولي

Prevalence of Theileria equi in horses of Varamine's area by staining method and molecular method

ﺗﯿﻠﺮﯾــﺎ اﮐــﻮﺋﯽ ﺗﮏﯾﺎﺧﺘــﻪاي از ﺷــﺎﺧﻪ اﭘــﯽﮐﻤﭙﻠﮑﺴــﺎ و ﺧــﺎﻧﻮاده ﺗﯿﻠﺮﯾــﺪه ﻣﯽﺑﺎﺷــﺪ ﮐــﻪ در ﮔﺬﺷــﺘﻪ ﺑﺎﺑﺰﯾــﺎ اﮐــﻮﺋﯽ ﻧﺎﻣﯿــﺪه ﻣـﯽﺷـﺪ. اﯾﻦ اﻧﮕـﻞ ﻋﺎﻣـﻞ اﺻـﻠﯽ ﺑﯿﻤـﺎري ﭘﯿﺮوﭘﻼﺳـﻤﻮز اﺳـﺐ ﺳـﺎﻧﺎن )اﺳـﺐ، اﻻغ، ﮔـﻮرﺧﺮ و ﻗـﺎﻃﺮ( اﺳـﺖ. اﯾـﻦ ﺑﯿﻤـﺎري ﻧـﻪ ﺑﻪ ﺧﺎﻃﺮ ﮔﺴـﺘﺮش ﺳـﺮﯾﻊ و اﺛـﺮات ﻣﺨـﺮب ﺑـﺮ روي اﺳـﺐﻫـﺎ و ﻣـﺮگ و ﻣﯿـﺮ در ﻣـﻮارد ﺣـﺎد ﺑﻠﮑـﻪ ﺑـﻪ ﻋﻠـﺖ ﺿـﺮر اﻗﺘﺼـﺎدي ﻧﺎﺷﯽ از اﯾﺠـﺎد ﻣﺤـﺪودﯾﺖﻫـﺎي ﺗﺠـﺎري و ﻣﻤﻨﻮﻋﯿـﺖﻫـﺎي ﻣﮑـﺎﻧﯽ در ﻣﻨـﺎﻃﻘﯽ ﮐـﻪ ﺷـﯿﻮع ﻣﯽﯾﺎﺑـﺪ ﻣـﻮرد ﺗﻮﺟـﻪ ﻗـﺮار ﮔﺮﻓﺘـﻪ اﺳــﺖ. در اﯾــﻦ ﻣﻄﺎﻟﻌــﻪ از 100 ﻧﻤﻮﻧــﻪ ﺧــﻮن از اﺳــﺐﻫــﺎي ﭼﻬــﺎر ﻣﻨﻄﻘــﻪ ﻣﺨﺘﻠــﻒ در وراﻣــﯿﻦ ﺟﻤــﻊآوري ﺷــﺪ و ﺳــﭙﺲ ﺑــﺎ روش رﻧﮓآﻣﯿـﺰي و ﻣﻮﻟﮑـﻮﻟﯽ از ﻧﻈـﺮ آﻟـﻮدﮔﯽ ﻣـﻮرد ﺑﺮرﺳـﯽ ﻗـﺮار ﮔﺮﻓـﺖ ﻫﻤﭽﻨـﯿﻦ وﺟـﻮد آﻟـﻮدﮔﯽ در ﮔـﺮوهﻫـﺎي ﻣﺨﺘﻠـﻒ ﺳــﻦ، ﺟــﻨﺲ، ﻧــﮋاد، ﻧــﻮع ﻧﮕﻬــﺪاري و ﻓﻌﺎﻟﯿــﺖ ﺑــﺪﻧﯽ در ﻧــﺮم اﻓــﺰار Excel 2010 ﺟﻤــﻊآوري و ﻃﺒﻘــﻪﺑﻨــﺪي ﺷــﺪ و ﺳــﭙﺲ ﺑــﺎ ﻧــﺮم اﻓــﺰار SPSS ﺑــﺎ ﺷــﻤﺎره ورژن 25 و اﺳــﺘﻔﺎده از آزﻣــﻮن ﻣﺮﺑــﻊ ﮐــﺎي ﺑــﻪ ﺗﺠﺰﯾــﻪ و ﺗﺤﻠﯿــﻞ ﻧﺘــﺎﯾﺞ ﭘﺮداﺧﺘــﻪ ﺷــﺪ. در اﯾــﻦ ﻣﻄﺎﻟﻌﻪ 7 درﺻﺪ آﻟﻮدﮔﯽ وﺟﻮد داﺷﺖ ﮐﻪ ﻟﺰوم ﺗﻮﺟﻪ ﺑﻪ اﯾﻦ ﺑﯿﻤﺎري را ﺑﺮاي داﻣﭙﺰﺷﮑﺎن ﻧﺸﺎن ﻣﯽدﻫﺪ.

Theileria equi is a protozoan of the Apicomplexa phylum and the family Theileridae, before known as Babesia equi. This parasite is the main cause of equine piroplasmosis (horses, donkeys, zebras and mules). The disease has been noted not because of its rapid spread and devastating effects on horses and mortality in acute cases, but because of the economic losses caused by trade restrictions and limitations in areas where it is prevalent. In this study, blood samples were collected from 100 horses from four different regions in Varamin and then its result was investigated from the perspective of infection by stanining and molecular methods. Furthermore, the presence of infection in different groups including age, sex, breed, type of maintenance and bodily activity was collected and classified with the assistance of Excel 2010 software and afterwards we analyzed the data with SPSS software by version number 25 and also using chi-square test. Finally, we concluded that there was 7% contamination, which demonstrates that paying attention to this disease would be essential for veterinarians.