پديد آورندگان :
ﭘﻮرﻣﻬﺪي، اوﯾﺲ داﻧﺸﮕﺎه آزاد اﺳﻼﻣﯽ واﺣﺪ ﺑﺎﺑﻞ - ﮔﺮوه داﻣﭙﺰﺷﮑﯽ، ﺑﺎﺑﻞ، اﯾﺮان , ﻣﺸﺘﺎق، اﯾﻼر داﻧﺸﮕﺎه آزاد اﺳﻼﻣﯽ واﺣﺪ شبستر - ﮔﺮوه داﻣﭙﺰﺷﮑﯽ، شبستر، اﯾﺮان , رﺿﻮان، ﻣﻬﺴﺎ داﻧﺸﮕﺎه آزاد اﺳﻼﻣﯽ واﺣﺪ شهركرد، شهركرد، اﯾﺮان , ﻏﻼﻣﯽ آﻫﻨﮕﺮان، ﻣﺠﯿﺪ داﻧﺸﮕﺎه آزاد اﺳﻼﻣﯽ واﺣﺪ شهركرد - علوم باليني، شهركرد، اﯾﺮان
كليدواژه :
ﺗﺮﻣﯿﻢ زﺧﻢ , ﻋﺴﻞ ﺑﻬﺎر ﻧﺎرﻧﺞ , ﻓﻨﯽﺗﻮﺋﯿﻦ , زﺧﻢ ﺑﺮﺷﯽ , ﻣﻮش ﺻﺤﺮاﺋﯽ
چكيده فارسي :
در اﯾﻦ ﻣﻄﺎﻟﻌﻪ ﺗﺠﺮﺑﯽ 32 ﻣﻮش ﺻﺤﺮاﺋﯽ ﻧﯿﻤﻪ ﺑﯿﻬﻮش ﺷﺪﻧﺪ و زﺧﻤﯽ ﺗﻤﺎم ﺿﺨﺎﻣﺖ در ﻗﺴﻤﺖ ﭘﺸﺘﯽ ﺑﻪ اﺑﻌﺎد ﯾﮏ ﺳﺎﻧﺘﯽﻣﺘﺮ ﻣﺮﺑﻊ اﯾﺠﺎد ﺷﺪ. ﻣﻮشﻫﺎ در ﮔﺮوهﻫﺎي ﮐﻨﺘﺮل ﻣﻨﻔﯽ، ﮐﻨﺘﺮل ﻣﺜﺒﺖ )او ﺳﺮﯾﻦ(، ﻋ ﺴﻞ ﺑﻬﺎر ﻧﺎرﻧﺞ 75 در ﺻﺪ و ﻓﻨﯽﺗﻮﺋﯿﻦ 1 در ﺻﺪ ﺗﻘ ﺴﯿﻢﺑﻨﺪي ﺷﺪﻧﺪ. در ﮔﺮوه ﺗﺠﺮﺑﯽ ﺗﺤﺖ درﻣﺎن روزاﻧﻪ 2 ﺑﺎر ﺑﻪ ﻣﻘﺪار ﯾﮏ ﮔﺮم از ﭘﻤﺎد ﺑﻪ ﺻﻮرت ﯾﮏ ﻻﯾﻪ ﻧﺎزك ﺑﺮ روي ﺳﻄﺢ زﺧﻢﻫﺎ ﺑﻪ ﻣﺪت 14 روز ﻗﺮار داده ﺷﺪ. ﻧﻤﻮﻧﻪﺑﺮداري ﺟﻬﺖ ﺑﺮر ﺳﯽﻫﺎي ﻫﯿ ﺴﺘﻮﭘﺎﺗﻮﻟﻮژي در روزﻫﺎي 10 ،7 ،4 و 14 ﺑﻌﺪ از اﯾﺠﺎد زﺧﻢ ﺻــﻮرت ﮔﺮﻓﺖ. ﺑﺮاي اﻧﺪازهﮔﯿﺮي ﺟﻤﻊﺷــﺪﮔﯽ زﺧﻢ از ﻧﺮماﻓﺰار دﯾﺠﯽ ﻣﺎﯾﺰر اﺳــﺘﻔﺎده ﺷــﺪ. در اﯾﻦ ﺑﺮرﺳــﯽ ﻣﯿﺰان آﻧﮋﯾﻮژﻧﺰ، ﻓﯿﺒﺮوﭘﻼزي، ﺗﺸــﮑﯿﻞ ﺑﺎﻓﺖ ﭘﻮﺷــﺸــﯽ، رﺳــﻮب ﮐﻼژن و ارﺗﺸــﺎح ﺳــﻠﻮلﻫﺎي اﻟﺘﻬﺎﺑﯽ ﻣﻮرد ارزﯾﺎﺑﯽ ﻗﺮار ﮔﺮﻓﺖ. در ﺑﺮرﺳــﯽ ﻣﯿﮑﺮوﺳــﮑﻮﭘﯿﮏ در روز 4، آﺳــﯿﺐ اﭘﯿﺪرم و درم ﺑﺎ ﺣﻀــﻮر ﻟﺨﺘﻪ ﺧﻮﻧﯽ و ﻧﻔﻮذ ﺳــﻠﻮلﻫﺎي اﻟﺘﻬﺎﺑﯽ در ﮔﺮوه ﺗﺤﺖ درﻣﺎن و ﺑﺎﻓﺖ ﺟﻮاﻧﻪاي در ﺣﺎل ﺗﺸﮑﯿﻞ در ﮔﺮوهﻫﺎي ﻋﺴﻞ ﺑﻬﺎر ﻧﺎرﻧﺞ 75 درﺻﺪ و ﻓﻨﯽﺗﻮﺋﯿﻦ 1 درﺻﺪ ﻣﺸﺎﻫﺪه ﺷﺪ. در روز ﻫﻔﺘﻢ ﺑﺎﻓﺖ ﺟﻮاﻧﻪاي در ﮔﺮوهﻫﺎي ﺗﺤﺖ درﻣﺎن ﻗﺎﺑﻞ ﺗﺸﺨﯿﺺ ﺑﻮد. در ﮔﺮوه ﺗﺤﺖ درﻣﺎن ﺑﺎ ﻋﺴﻞ 75 درﺻﺪ ﺗﺮاﮐﻢ ﺳﻠﻮﻟﯽ ﺑﺎﻓﺖ ﺟﻮاﻧﻪاي ﻧﺴﺒﺖ ﺑﻪ ﺳﺎﯾﺮ ﮔﺮوهﻫﺎ ﮐﻤﺘﺮ و ﺳﻨﺘﺰ ﮐﻼژن ﻧﺴﺒﺖ ﺑﻪ ﺳﺎﯾﺮ ﮔﺮوهﻫﺎ ﺑﯿﺸﺘﺮ و ﺑﺎﻓﺖ ﭘﻮﺷﺸﯽ در ﺣﺎل ﺗﺸﮑﯿﻞ ﻧﯿﺰ ﻗﺎﺑﻞ ﺗﺸﺨﯿﺺ ﺑﻮد. در روز دﻫﻢ از ﺗﺮاﮐﻢ ﺳﻠﻮلﻫﺎي آﻣﺎ ﺳﯽ، ﻋﺮوق ﺧﻮﻧﯽ و ﺳﻠﻮلﻫﺎي ﻓﯿﺒﺮوﺑﻼ ﺳﺘﯽ در ﺑﺎﻓﺖ ﺟﻮاﻧﻪاي ﮐﺎ ﺳﺘﻪ ﺷﺪ. در ﮔﺮوه ﮐﻨﺘﺮل ﻣﻨﻔﯽ و ﮐﻨﺘﺮل ﻣﺜﺒﺖ ﻧﺴﺒﺖ ﺑﻪ ﻋﺴﻞ 75 درﺻﺪ و ﻓﻨﯽﺗﻮﺋﯿﻦ 1 درﺻﺪ ﺑﺎﻓﺖ ﺟﻮاﻧﻪاي ﺟﻮانﺗﺮ ﺑﻪ ﻧﻈﺮ ﻣﯽرﺳﯿﺪ. ﺑﺎﻓﺖ ﺟﻮاﻧﻪاي در ﮔﺮوه ﻋﺴﻞ 75 در ﺻﺪ و ﻓﻨﯽﺗﻮﺋﯿﻦ 1 در ﺻﺪ ﺗﺮاﮐﻢ ﺳﻠﻮﻟﯽ ﮐﻤﺘﺮ و ﺳﻨﺘﺰ ﮐﻼژن ﺑﯿ ﺸﺘﺮ را ﻧ ﺸﺎن داد. در ﮔﺮوه ﻋ ﺴﻞ 75 در ﺻﺪ ﺗ ﺸﮑﯿﻞ ﺑﺎﻓﺖ ﭘﻮ ﺷ ﺸﯽ در ﻣﻘﺎﯾ ﺴﻪ ﺑﺎ روز ﻫﻔﺘﻢ ﭘﯿ ﺸﺮﻓﺖ ﺑﯿ ﺸﺘﺮي دا ﺷﺖ. در روز ﭼﻬﺎردﻫﻢ در ﮔﺮوه ﻋ ﺴﻞ 75 در ﺻﺪ ﺑﺎﻓﺖ ﺟﻮاﻧﻪاي ﺑﺎﻟﻎ ﺑﻪ ﺻﻮرت ﺗﺮاﮐﻢ ﺳﻠﻮﻟﯽ ﮐﻢ و رﺷﺘﻪﻫﺎي ﮐﻼژن ﺿﺨﯿﻢ و ﻣﻨﻈﻢ و ﺑﺎﻟﻎﺗﺮ از ﺳﺎﯾﺮ ﮔﺮوهﻫﺎ ﺑﻪ ﻧﻈﺮ ﻣﯽرﺳﯿﺪ و ﺑﺎﻓﺖ ﭘﻮﺷﺸﯽ ﺑﻪﺻﻮرت ﮐﺎﻣﻞ ﺳﻄﺢ زﺧﻢ را ﭘﻮ ﺷﺎﻧﺪه ﺑﻮد. در ﺑﺮر ﺳﯽ اﻧﺪازهﮔﯿﺮي اﻧﻘﺒﺎض زﺧﻢ ﻧﺘﺎﯾﺞ ﺣﺎﮐﯽ از آن ﺑﻮد ﮐﻪ ﮔﺮوه ﺗﺤﺖ درﻣﺎن ﺑﺎ ﻋ ﺴﻞ 75 درﺻﺪ ﻧﺴﺒﺖ ﺑﻪ ﻓﻨﯽﺗﻮﺋﯿﻦ 1 درﺻﺪ ﺑﻬﺒﻮدي ﺑﺎﻻﺗﺮي داﺷﺘﻪ ﮐﻪ اﯾﻦ اﺧﺘﻼف از ﻟﺤﺎظ آﻣﺎري ﻣﻌﻨﯽدار ﺑﻮد.
چكيده لاتين :
In this experimental study, 32 rats were anesthetized with 2% ketamine and 5% xylazine, ip. Then a shear wound was created on the back of each animal, measuring one cm2 in full thickness. Mice were divided into negative control, positive control (osrine), spring orange 75% and phenytoin 1% groups. In the experimental group, 2 g of ointment was applied twice a day as a thin layer on the surface. Wounds were monitored for 14 days. Sampling was performed for histopathological examinations on days 4, 7, 10 and 14 after wounding. Digiser software was used to measure wound shrinkage. In this study, the rate of angiogenesis, fibroplasia, epithelial formation, collagen deposition and infiltration of inflammatory cells were evaluated. On microscopic examination on day 4, epidermal and dermal damage was observed in the presence of blood clots and infiltration of inflammatory cells in the treated group and budding tissue forming in the 75% spring orange and phenytoin 1% honey groups. On day 7, bud tissue was detectable in the treated groups. In the group treated with honey, 75% of the cell density of bud tissue was lower than the other groups and collagen synthesis was higher than the other groups and the developing epithelial tissue was also detectable. On day 10, the density of inflammatory cells, blood vessels, and fibroblasts in bud tissue decreased. In the negative and positive control groups, 75% of the bud tissue appeared younger than honey and 1% phenytoin. Germ tissue in honey group 75% and phenytoin 1% showed lower cell density and higher collagen synthesis. In the honey group, 75% of the formation of epithelial tissue was more advanced compared to the 7th day. On the 14th day in the honey group, 75% of the mature bud tissue appeared to have low cell density and collagen fibers were thicker, regular and more mature than the other groups, and the covering tissue completely covered the wound surface. In the study of wound contraction, the results showed that the group treated with honey 75% had a higher improvement than phenytoin 1%, which was statistically significant.
