در ايران B اولين گزارش از وجود گونه MTDNA PCR-RELP كمپلكس آنوفل كوليسيفاسيس مهمترين ناقل مالاريا در منطقه بلوچستان به روش B و A تشخيص گونه هاي

Detecting the Anopheles culicifacies complex, species A and B in Baluchestan using mtDNA PCRRFLP assay; the first report of species B from Iran

Introduction: Mosquitoes of the Anopheles culicifacies complex are one of the main malaria vectors in Indian subcontinent and Middle East countries as well as in Iran. Based on cytological characteristcs there are five morphologically indistinguishable species in this complex that called A, B, C, D and E. These species have got different biological and behaviour characteristics, and show different vectorial capacity. Methods: Due to problems inherent in the cytological studies a DNA based method mtDNA PCR-RFLP was conducted to study genetic variation and itʹs utility in identifying species composition of the complex in Baluchestan district of Iran. A 1512 bp mtDNA of cytochrome oxidase subunit I and II (COI-COIL) or an 875 bp mtDNA of COI were amplified and treated by different restriction enzymes (PCR-RFLP). Results: Results of this study showed that at least two species, A and B, of the complex are present in the region. This is the first report on the occurrence of species B in Iran. In addition to species A and B, there were some other haplotypes within the specimens which represent either the occurrence of other members of the complex (persumably species E) or intraspecies variation. Conclusion: These results are very important in epidemiology of malaria in the region and will be remain to be confirmed by further cytological and molecular works.