سنتز اكسي توسين از آلفاآمينو اسيد در فاز جامد

Synthesis of oxytocin from a-amino acids in solid phase

Oxytocin is excreted from the posterior pituitary gland and has many physiological properties like uterine contraction and consequently facilitating labour. This peptide amide is prepared by chemical synthetic means and has pharmaceutical applications in human and domestic animals. There are two methods of preparation of peptides, solution and solid phase; the latter being used in this study. The resin selected was Rink Amide MBHA and the alpha - amino acid, Fmoc-Gly-OH, was bonded to the resin followed by deprotection of Fmoc group by 20% piper idine.The next amino acid was added and this was continued till the chain was completed. The synthesized peptide was detached from the resin under acidic condition and then precipitated. The disulphide bridge between Cysteine 1 and 6 was achieved using iodine. The purified product was analyzed with respect to its purity, amino acids and biological activity. The results obtained were compared with the standard sample. The purity of the product was 95% and amino acid analysis showed the presence of all amino acids in the hormone. Biological activity was 467.05 unit per milligram.