A اثر سولفات منيزيم بر تندش اسپور، رشد و توليد توكسين از كلستريديوم بوتولينوم تيپ

The effect of magnesium sulfate on spore germination, outgrowth and toxin production of Clostridium botulinum type A

The principal hazard of clostridia in human is production of the most lethal toxin. In order to prevent food poisoning and botulism infection, developing new methodʹs to control bacterial growth, toxin production and its activation is essential. Recently, the hotulinum toxin type A has been considered in treatment of different disease and many efforts have been carried out to. find out its production and stability mechanisms. In this investigation, the effects of magnesium sulfate, and incubation time on germination of spore, growth and toxin poduction by Clostridium botulinum type A were studied. Samples with different concentrations of magnesium sulfate were inoculated with 10^6 cfu/ml spores, or with 10^7 cfu/ml bacterial suspension, anaerobically incubated at 35 ° C, analysed for spore germination, growth and toxin production. The results indicated that, magnesium sulfate at the level of 6 mg/ml increases the rate of Clostridium botulinum type A spore germination about 40 prevent and at 8 mg/ml, increases the growth, protein production, and neurotoxicity of the bacteria 4, 2 and 100 times, respectively. The results of this study show that, enzymes affect spore germination and metabolism can he induced activil}% by magnesium ions. Therefore, the rate of spore germination and total protein production is increased. It may be possible that, magnesium ions have been chelated with toxin and alert its configuration, then the neurotoxicity increased.