در مدل حيواني A توليد شده عليه سم پوتولينوم تايپ )IGY( Y ارزيابي اثر آنتي بادي

Evaluation of antibody Y (IgY) raised against botulinum neurotoxin type A in an animal model

Introduction: The yolk of eggs laid by immunized chicken has been recognized as an important source of polyclonal antibodies. Specific antibodies produced in chicken offer several important advantages over producing antibodies in mammals. This antibody can be considered as a real alternative to the traditional antibody production in laboratory animals. The purpose of this study was to explore the influence of passive oral administration of antibody to botulinum neurotoxin type A in mouse model for therapeutic usage. Methods: Chicken antibodies were obtained from the yolk eggs of chickens immunized with botulinum neurotoxin. Immunization was done with 20,ug of toxoid in incomplete ferunds adjuvant per chicken injected into breast muscle of lay. Antibody extracted from egg yolks by biochemical methods in two steps and ultracentrfugation methods. Preimmune chicken egg yolk cantaining immunoglobulin Y (IgY antibody) to C. botulinum neurotoxin was supplied in drinking water of mice one day before experiment. Then, all animals were challenged with C. botulinum neurotoxin. Mice were controlled during 120 hrs. Results: Each yolk, after extraction contains about 40 mg /ml of Ig Y and specific antibodies was 20 mg/ml after purification. Our results indicated that antibody could protect mice against botulinum neurotoxin. Conclusion: This is the first demonstration that IgY raised against C. botulinum neurotoxin can have a protective effect against C. botulinum toxin.