فلوسيتومتري ، روشي سريع و دقيق براي افتراق هليكوباكترپيلوري زنده از مرده

Flow cytometry: A reliable and rapid method for differentiation of living and dead Helicobacter

It is about 20 years that Helicobacter pylori has been discovered as an etiologic factor for peptic ulcer and gastric adenocarcinoma and lymphoma. The bacterium has a spiral form, but under stress conditions can transform to coccoid form. Coccoids are not culturable (by routine methods) therefore it is believed that, they are the morphologic manifestations of cell death. Some investigators successfully showed that coccoids are alive cells. In this research, using flow cytometry, we were able to discriminate between dead and alive cells. The results of this study showed that all of the reserved bacteria in water converted to the coccoid form after 24 hours, also after conversion to the coccoid form, the bacteri can live until 11 months under low temperature conditions. The technique is reliable, fast and convenient to investigate the effect of different environmental factors on H pylori viability.