ﺑﺮرﺳﯽ اﺛﺮ ﺗﺮاﻣﺎدول ﺑﺮ ﺑﺎﻓﺖ ﻃﺤﺎل در ﻣﻮش ﻫﺎي ﺻﺤﺮاﯾﯽ رت ﻧﺮ ﻧﮋاد وﯾﺴﺘﺎر

The effect of tramadol on spleen tissue in male Wistar rats

ﭼﮑﯿﺪه ﺗﺮاﻣﺎدول داروﺋﯽ ﺿﺪ درد ﺑﺎ اﺛﺮ ﻣﺮﮐﺰي ﺑﻮده ﮐﻪ از دﺳﺘﻪ داروﻫﺎي ﻣﺴﮑﻦ ﻣﺨﺪر ﯾﺎ اﭘﻮﺋﯿﺪﻫﺎي ﻣﺼﻨﻮﻋﯽ ﻣﯽ ﺑﺎﺷﺪ. ﻫﺪف از اﯾﻦ ﺗﺤﻘﯿﻖ ﺑﺮرﺳﯽ اﺛﺮ ﻣﺼﺮف ﻃﻮﻻﻧﯽ ﻣﺪت ﺗﺮاﻣﺎدول ﺑﺮ ﺑﺎﻓﺖ ﻃﺤﺎل در ﻣﻮش ﻫﺎي ﺻﺤﺮاﯾﯽ رت ﻧﺮ ﻧﮋاد وﯾﺴﺘﺎر اﺳﺖ. در اﯾﻦ ﺗﺤﻘﯿﻖ 60 ﺳﺮ ﻣﻮش ﺻﺤﺮاﯾﯽ رت ﻧﺮ ﻧﮋاد وﯾﺴﺘﺎر ﺑﻪ 3 ﮔﺮوه )ﮐﻨﺘﺮل، ﺗﺠﺮﺑﯽ1،2( ﺗﻘﺴﯿﻢ ﮔﺮدﯾﺪ، ﮔﺮوه ﮐﻨﺘﺮل ﮐﻪ ﺑﺎﻓﺮ ﺳﯿﺘﺮات را ﺑﺼﻮرت درون ﺻﻔﺎﻗﯽ درﯾﺎﻓﺖ ﮐﺮدﻧﺪ، ﮔﺮوه ﺗﺠﺮﺑﯽ ﯾﮏ، ﺗﺮاﻣﺎدول را ﺑﺎ دوز 50 ﻣﯿﻠﯽ ﮔﺮم ﺑﺮ ﮐﯿﻠﻮ ﮔﺮم و ﮔﺮوه ﺗﺠﺮﺑﯽ دوم 100 ﻣﯿﻠﯽ ﮔﺮم ﺑﺮ ﮐﯿﻠﻮ ﮔﺮم ﺑﺼﻮرت روزاﻧﻪ ﺗﺎ 30 روز ﺗﺮاﻣﺎدول را درﯾﺎﻓﺖ ﮐﺮدﻧﺪ. ﺑﻌﺪ از ﭘﺎﯾﺎن روز ﻣﻘﺮر ﻧﻤﻮﻧﻪ ﻫﺎ ﺑﺮاي ﺑﺮرﺳﯽ ﻫﯿﺴﺘﻮﻟﻮژي ﺑﺎﻓﺖ ﻃﺤﺎل و ﺑﺮﺧﯽ ﭘﺎراﻣﺘﺮﻫﺎي ﺧﻮﻧﯽ، ﻣﻮرد آزﻣﺎﯾﺶ ﻗﺮار ﮔﺮﻓﺘﻨﺪ. درﮔﺮوه ﻫﺎي ﺗﺠﺮﺑﯽ ﯾﮏ و دو ﺗﺮاﮐﻢ ﺑﺎﻓﺘﯽ ﺑﺴﯿﺎر زﯾﺎد و ﻓﻀﺎي ﺳﯿﻨﻮزوﺋﯿﺪ ﭘﺮ از ﺳﻠﻮل ﻫﺎي ﻟﻨﻔﺎوي و ﺧﻮﻧﯽ ﻣﯽ ﺑﺎﺷﺪ. ﻣﯿﺰان ذرات ﻫﻤﻮﺳﯿﺪرﯾﻦ در ﻫﺮ دو ﮔﺮوه ﺗﺠﺮﺑﯽ اﻓﺰاﯾﺶ ﻗﺎﺑﻞ ﺗﻮﺟﻬﯽ را ﻧﺸﺎن ﻣﯽ دﻫﺪ. اﯾﻦ ﺗﺤﻘﯿﻖ ﻧﺸﺎن داد ﮐﻪ ﻣﺼﺮف ﺧﻮراﮐﯽ دراز ﻣﺪت ﺗﺮاﻣﺎدول ﺑﺎﻋﺚ ﺗﻐﯿﯿﺮات ﺑﺎﻓﺘﯽ ﻃﺤﺎل ﻣﯽ ﮔﺮدد.

Tramadol the right drugs are synthetic opioid analgesics or Epoxied. The aim of this study was to evaluate the effect of oral tramadol on Spleen in rats Wistar male rats. In this study, 60 male Wistar rats randomized into 3 groups (1 control group, and experimental groups of 1, 2). In control group adequate serum physiology and in experimental groups a dose of mercuric chloride infused into peritoneal cavity, for 30 days. The amount of gavage tramadol were, 50ml /kg group 2, 100 ml /kg , every other day for 30 days. After the end of the day appointed for Histological analysis some blood parameters of Spleen samples, were tested. In experimental groups one and two, tissue density is very high and the sinusoidal space is full of lymph and blood cells. The amount of hemosiderin particles in both experimental groups showed a significant increase. This study showed that long-term oral administration of tramadol causes splenic tissue changes.