ﻧﺎﻧﻮ رﯾﺰﭘﻮﺷﺎﻧﯽ ﭘﭙﺘﯿﺪﻫﺎي زﯾﺴﺖﻓﻌﺎل ﺣﺎﺻﻞ از آﺑﮑﺎﻓﺖ آﻧﺰﯾﻤﯽ ﺿﺎﯾﻌﺎت ﻣﯿﮕﻮ ﺑﺎ ﭘﻮﺷﺶ ﺗﺮﮐﯿﺒﯽ ﻧﺎﻧﻮﻟﯿﭙﻮ زوم - ﮐﯿﺘﻮزان و ارزﯾﺎﺑﯽ ﻓﻌﺎﻟﯿﺖ ﺿﺪ ﺑﺎﮐﺘﺮﯾﺎﯾﯽ، آﻧﺘﯽاﮐﺴﯿﺪاﻧﯽ و ﺿﺪ ﻓﺸﺎر ﺧﻮﻧﯽ ﻣﺤﺼﻮل

Nanoencapsulation of bioactive peptides from shrimp wastes enzymatic hydrolysis with combined coating of nanoliposome-chitosan and evaluation of antibacterial, antioxidant and antihypertensive activity of the product

هدف از مطالعه حاضر ارزيابي خواص مختلف پپتيدهاي زيست‌‌فعال (با وزن كمتر از 3 كيلودالتون) نانوريزپوشاني‌‌شده با پوشش تركيبي نانوليپوزوم و كيتوزان بود. به همين جهت، ضايعات مراكز فراوري و بسته‌‌بندي ميگو با استفاده از آنزيم نئوتراز آبكافت و خواص ضدباكتريايي، آنتي‌‌اكسيداني و ضد فشار خوني محصول در قالب پنج تيمار شامل P (پپتيدهاي آزاد)، NP (نانوليپوزوم‌‌هاي حامل پپتيد)، NP-CH-0.05 (نانوليپوزوم‌‌هاي حامل پپتيد با پوشش 0/05 درصد كيتوزان)، NP-CH-0.01 (نانوليپوزوم‌‌هاي حامل پپتيد با پوشش 1/0 درصد كيتوزان) و NP-CH-0.5 (نانوليپوزوم‌‌هاي حامل پپتيد با پوشش 5/0 درصد كيتوزان) ارزيابي شد. نتايج نشان داد، همه تيمارهاي تحقيق تا حد مشخصي خواص ضد باكتريايي، آنتي‌‌اكسيداني و ضد فشار خوني دارند. تيمارهاي P و NP در هر سه خاصيت، مقادير تقريباً برابري ارائه كردند و خواص آنها اختلاف معني‌‌داري نداشت (0/05

The aim of this study was to evaluate the different properties of bioactive peptides (weight less than 3 kDa) nanoencapsulated with a combined coating of nanoliposome and chitosan. Accordingly, the wastes of shrimp processing and packaging centers were hydrolyzed using Neuterase enzyme and the antibacterial, antioxidant and antihypertensive properties of the product were evaluated in the form of five treatments including P (free peptides), NP (peptide-carrying nanoliposomes), NP-CH-0.05 (peptide-carrying nanoliposomes with a coating of 0.05% chitosan), NP-CH-0.1 (peptide-carrying nanoliposomes with a coating of 0.1% chitosan) and NP-CH-0.5 (peptide-carrying nanoliposomes with a coating of 0.5% chitosan). The results showed that all research treatments have certain antibacterial, antioxidant and antihypertensive properties. P and NP treatments provided almost equal values for all three properties and their properties did not differ significantly (p>0.05). Chitosancoated treatments had significantly more antibacterial, antioxidant and antihypertensive properties than P and NP treatments (p<0.05). In chitosan-coated treatments, with increasing the amount of chitosan in the coating, all three mentioned properties were significantly increased (p<0.05). Among the research treatments, the highest growth inhibition activity of Escherichia coli, Bacillus cereus and Staphylococcus aureus (45.89±0.96, 34.82±0.75 and 49.67±1.45%, respectively), the maximum amount of inhibition of DPPH, ABTS and linoleic acid peroxidation (63.52±2.86, 73.91±0.29 and 89.06±2.11%, respectively) and also the most effective antihypertensive property (37.91±1.29%) were related to NP-CH-0.5 treatment.