پديد آورندگان :
ﻓﺮزان، ﺣﺪﯾﺜﻪ داﻧﺸﮕﺎه آزاد اﺳﻼﻣﯽ واﺣﺪ داﻣﻐﺎن - داﻧﺸﮑﺪه ﻋﻠﻮم ﭘﺎﯾﻪ - ﮔﺮوه ﻣﯿﮑﺮوﺑﯿﻮﻟﻮژي، داﻣﻐﺎن، اﯾﺮان , ﻓﻀﻠﯽ، ﻣﮋﮔﺎن داﻧﺸﮕﺎه ﻋﻠﻮم ﭘﺰﺷﮑﯽ ﺷﺎﻫﺮود - داﻧﺸﮑﺪه ﭘﺰﺷﮑﯽ، ﺷﺎﻫﺮود، اﯾﺮان , ﻃﻬﻤﺎﺳﺒﯽ، ﺣﺎﻣﺪ داﻧﺸﮕﺎه ﻋﻠﻮم ﭘﺰﺷﮑﯽ ﺷﺎﻫﺮود - داﻧﺸﮑﺪه ﭘﺰﺷﮑﯽ، ﺷﺎﻫﺮود، اﯾﺮان , ﻧﻮروزي، ﭘﯿﺮاﺳﺘﻪ داﻧﺸﮕﺎه ﻋﻠﻮم ﭘﺰﺷﮑﯽ ﺷﺎﻫﺮود - داﻧﺸﮑﺪه ﭘﺰﺷﮑﯽ، ﺷﺎﻫﺮود، اﯾﺮان , ﻧﺼﯿﺮي، ﻣﺠﺘﺒﯽ داﻧﺸﮕﺎه ﻋﻠﻮم ﭘﺰﺷﮑﯽ ﺷﺎﻫﺮود - داﻧﺸﮑﺪه ﭘﺰﺷﮑﯽ، ﺷﺎﻫﺮود، اﯾﺮان , ﻣﯿﺮزاﯾﯽ، ﻣﻬﺪي داﻧﺸﮕﺎه ﻋﻠﻮم ﭘﺰﺷﮑﯽ ﺷﺎﻫﺮود - داﻧﺸﮑﺪه ﭘﺰﺷﮑﯽ، ﺷﺎﻫﺮود، اﯾﺮان
كليدواژه :
واﮐﻨﺶ زﻧﺠﯿﺮه اي ﭘﻠﯽ ﻣﺮاز , اﺳﺘﺮﭘﺘﻮﮐﻮك آﮔﺎﻻﮐﺘﯿﻪ , ﻣﻘﺎوﻣﺖ ﺑﻪ دارو , زﻧﺎن ﺑﺎردار
چكيده فارسي :
ﯾﮑﯽ از ﻋﻮاﻣﻞ ﻋﻔﻮﻧﺖ زا در زﻧﺎن ﺑﺎردار و ﻧﻮزادان ﺗﺎزه ﻣﺘﻮﻟﺪ ﺷﺪه، اﺳﺘﺮﭘﺘﻮﮐﻮك آﮔﺎﻻﮐﺘﯿﻪ ﻣﯽ ﺑﺎﺷﺪ. ﮐﭙﺴﻮل اﯾﻦ ﺑﺎﮐﺘﺮي اﻫﻤﯿﺖ وﯾﮋه اي در ﺑﯿﻤﺎري زاﯾﯽ دارد. ﻫﺪف ازا ﯾﻦ ﻣﻄﺎﻟﻌﻪ، ﺑﺮرﺳﯽ اﻟﮕﻮي ﻣﻘﺎوﻣﺖ ﺑﻪ آﻧﺘﯽ ﺑﯿﻮﺗﯿﮏ و ﭘﺮاﮐﻨﺶ ﺗﺎﯾﭗ ﻫﺎي ﮐﭙﺴﻮﻟﯽ در اﯾﺰوﻟﻪ ﻫﺎي اﺳﺘﺮﭘﺘﻮﮐﻮك آﮔﺎﻻﮐﺘﯿﻪ ﺟﺪا ﺷﺪه از زﻧﺎن ﺑﺎردار و ﻧﻮزادان ﺗﺎزه ﻣﺘﻮﻟﺪ ﺷﺪه ﻣﯽ ﺑﺎﺷﺪ. ﻣﻮاد و روش ﻫﺎ: در اﯾﻦ ﻣﻄﺎﻟﻌﻪ ﺗﻮﺻﯿﻔﯽ- ﺗﺤﻠﯿﻠﯽ، ﺑﺎ اﺳﺘﻔﺎده از آزﻣﻮن ﻫﺎي ﺑﯿﻮﺷﯿﻤﯿﺎﯾﯽ، اﯾﺰوﻟﻪ ﻫﺎي اﺳﺘﺮﭘﺘﻮﮐﻮك آﮔﺎﻻﮐﺘﯿﻪ از از واژن و رﮐﺘﻮم زﻧﺎن ﺑﺎردار و ﮔﻮش و ﺑﯿﻨﯽ ﻧﻮزادان ﺗﺎزه ﻣﺘﻮﻟﺪ ﺷﺪه، ﺟﺪا ﺷﺪ. ﺗﺎﯾﭗ ﻫﺎي ﮐﭙﺴﻮﻟﯽ VII ، VI ، V ، Iv ، III ، II ، Ib ، Ia و VIII ﺑﻪ ﺗﺮﺗﯿﺐ ﺗﻮﺳﻂ ژن ﻫﺎي cps1bj ، ps1Ah ، cps7M ، cps6I ، cps5O ، cps4N ، cps1a/2/3I ، cps2k و cps8j ﺷﻨﺎﺳﺎﯾﯽ ﺷﺪﻧﺪ. ﺟﻬﺖ ﺗﮑﺜﯿﺮ ژن ﻫﺎ از روش واﮐﻨﺶ زﻧﺠﯿﺮه اي ﭘﻠﯽ ﻣﺮاز )PCR ( اﺳﺘﻔﺎده ﮔﺮدﯾﺪ. ﻧﺘﺎﯾﺞ: در اﯾﻦ ﻣﻄﺎﻟﻌﻪ، 73 اﯾﺰوﻟﻪ )52/8%( از ﻧﻤﻮﻧﻪ ﻫﺎي رﮐﺘﻮواژﯾﻨﺎل ﻣﺎدران ﺑﺎردار و 61 اﯾﺰوﻟﻪ )44/2%( از ﮔﻮش و ﺑﯿﻨﯽ ﻧﻮزادان ﺗﺎزه ﻣﺘﻮﻟﺪ ﺷﺪه ﺟﻤﻊ آوري ﮔﺮدﯾﺪ. در ﻧﻤﻮﻧﻪ ﻫﺎي رﮐﺘﻮواژﯾﻨﺎل، ﺳﻮﯾﻪ ﻫﺎي ﻣﻘﺎوم ﺑﻪ ﺗﺘﺮاﺳﺎﯾﮑﻠﯿﻦ )82/1%( و ارﯾﺘﺮوﻣﺎﯾﺴﯿﻦ )83/5%( ﺑﯿﺸﺘﺮﯾﻦ ﻓﺮاواﻧﯽ را داﺷﺘﻨﺪ. ﻫﻤﭽﻨﯿﻦ، در ﻧﻤﻮﻧﻪ ﻫﺎي ﺣﻠﻖ و ﺑﯿﻨﯽ، ﺳﻮﯾﻪ ﻫﺎي ﻣﻘﺎوم ﺑﻪ رﯾﻔﺎﻣﭙﯿﻦ )83/6%(، ارﯾﺘﺮوﻣﺎﯾﺴﯿﻦ )98/3%(، ﺗﺘﺮاﺳﺎﯾﮑﻠﯿﻦ )93/4%( و ﮐﻠﯿﻨﺪﻣﺎﯾﺴﯿﻦ )83/6%(، ﻓﺮاواﻧﯽ ﺑﺎﻻﯾﯽ را ﻧﺸﺎن دادﻧﺪ. در ﻫﺮ دو ﮔﺮوه، ﺗﺎﯾﭗ ﮐﭙﺴﻮﻟﯽ III )14/4%( و ﺑﻌﺪ از آن ﺑﻪ ﺗﺮﺗﯿﺐ ﺗﺎﯾﭗ ﻫﺎي ﮐﭙﺴﻮﻟﯽ Ib ،(%7/9) II )7/2%( و V )6/5%( ﺑﯿﺸﺘﺮﯾﻦ ﻓﺮاواﻧﯽ را داﺷﺘﻨﺪ. ﺗﺎﯾﭗ ﻫﺎي ﮐﭙﺴﻮﻟﯽ VIII ,VII Ia و VI در ﻫﯿﭻ ﯾﮏ از اﯾﺰوﻟﻪ ﻫﺎ ﺗﺸﺨﯿﺺ داده ﻧﺸﺪ. ﻓﺮاواﻧﯽ ﺗﺎﯾﭗ ﻫﺎي ﮐﭙﺴﻮﻟﯽ II ، Ib ، III و V اﻓﺰاﯾﺶ ﻣﻌﻨﯽ داري را در ﺳﻮﯾﻪ ﻫﺎي ﻣﻘﺎوم ﺑﻪ آﻧﺘﯽ ﺑﯿﻮﺗﯿﮏ ﻧﺸﺎن داد. ﻧﺘﯿﺠﻪ ﮔﯿﺮي: ﻧﺘﺎﯾﺞ ﻣﺎ ﻣﺸﺨﺺ ﮐﺮد ﮐﻪ ﻓﺮاواﻧﯽ ﺑﺎﻻي ﺗﺎﯾﭗ ﻫﺎي ﮐﭙﺴﻮﻟﯽ اﺳﺘﺮﭘﺘﻮﮐﻮك آﮔﺎﻻﮐﺘﯿﻪ در ﻣﺎدران ﺑﺎردار، ﻧﻘﺶ ﻣﻬﻤﯽ در اﻓﺰاﯾﺶ ﻣﻘﺎوﻣﺖ ﺑﻪ آﻧﺘﯽ ﺑﯿﻮﺗﯿﮏ ﻫﺎي ﻣﺨﺘﻠﻒ دارد.
چكيده لاتين :
Streptococcus agalactiae is one of the infectious agents in pregnant women and newborns. The capsule of
this bacterium is importance in pathogenesis. This study aimed to investigate antibiotic resistance patterns and distribution
of capsule types in S. agalactiae isolates collected from maternal-newborn.
Methods: First, using biochemical tests, S. agalactiae isolates were collected from the vagina and rectum of pregnant
women and the ears and nose of newborns. Capsule types Ia, Ib, II, III, Iv, V, VI, VII, and VIII were identified by the ps1Ah,
cps1bj, cps2k, cps1a / 2 / 3I, cps4N, cps5O, cps6I, cps7M and cps8j genes. Polymerase chain reaction (PCR) was used to
amplify the genes.
Results: In this study, 73 isolates (52.8%) were collected from rectovaginal, and 61 isolates (44.2%) were collected from
the ear and nose. In rectovaginal specimens, tetracycline (82.1%) and erythromycin (83.5%) resistant strains were most
common. Also, in nose and ear samples, resistance to rifampin (83.6%), erythromycin (98.3%), tetracycline (93.4%), and
clindamycin (83.6%) showed high frequency. In both groups, capsule type III (14.4%), followed by capsule type II (7.9%),
Ib (7.2%), and V (6.5%) had the highest frequency, respectively. Capsule types VIII, VII Ia, and VI were not detected in any
of the isolates. The frequency of capsule types III, Ib, II, and V significantly increased antibiotic-resistant strains.
Conclusion: Our results showed that the high frequency of S. agalactiae capsule types in pregnant women has an essential
role in increasing resistance to various antibiotics.
