ﺑﺮرﺳﯽ ﺧﻮاص آﻧﺘﯽ اﮐﺴﯿﺪاﻧﯽ و ﺿﺪ ﻣﯿﮑﺮوﺑﯽ ﻓﯿﻠﻢ ﻫﺎي ﻧﺎﻧﻮ ﮐﺎﻣﭙﻮزﯾﺘﯽ ﺣﺎوي ﻧﺎﻧﻮ ذرات ﻧﻘﺮه ﺳﻨﺘﺰي ﺑﺎ ﻋﺼﺎره ﮔﯿﺎه ﻧﻌﻨﺎع

Investigation of Antioxidant and Antimicrobial Properties of Nanocomposite Films Containing Synthesis of Silver Nanoparticles with Mentha extract

ﺑﺎ ﺗﻮﺟﻪ ﺑﻪ ﻣﺸﮑﻼت زﯾﺴﺖ ﻣﺤﯿﻄﯽ و اﻫﻤﯿﺖ آن، اﻣﺮوزه اﺳﺘﻔﺎده از ﻓﯿﻠﻢ ﻫﺎي ﻧﺎﻧﻮ ﮐﺎﻣﭙﻮزﯾﺘﯽ زﯾﺴﺖ ﺗﺨﺮﯾﺐ ﭘﺬﯾﺮ از اﻫﻤﯿﺖ وﯾﮋه اي ﺑﺮﺧﻮردار اﺳﺖ. در اﯾﻦ ﭘﮋوﻫﺶ ﻧﺎﻧﻮ ذرات ﻧﻘﺮه ﺑﺎ اﺳﺘﻔﺎده از ﻋﺼﺎره آﺑﯽ ﮔﯿﺎه ﻧﻌﻨﺎع ﺳﻨﺘﺰ و ﻓﻌﺎﻟﯿﺖ آﻧﺘﯽ اﮐﺴﯿﺪاﻧﯽ ﻧﺎﻧﻮ ذره ﻧﻘﺮه ﺑﯿﻮﺳﻨﺘﺰ ﺷﺪه ﺑﻪ روش ﺳﺒﺰ ﺑﺎ اﺳﺘﻔﺎده از ﻗﺎﺑﻠﯿﺖ ﺟﺬب رادﯾﮑﺎل ﻫﺎي 1،1- دﯾﻔﻨﯿﻞ2 ﭘﯿﮑﺮﯾﻞ-ﻫﯿﺪرازﯾﻞ و ﻓﻌﺎﻟﯿﺖ ﺿﺪ ﻣﯿﮑﺮوﺑﯽ ﺑﻪ روش ﺗﻌﯿﯿﻦ ﺣﺪاﻗﻞ ﻏﻠﻈﺖ ﻣﻬﺎرﮐﻨﻨﺪﮔﯽ، ﻣﻮرد ﺑﺮرﺳﯽ ﻗﺮار ﮔﺮﻓﺖ. ﺳﺎﺧﺘﺎر ﯾﮑﻨﻮاﺧﺖ و ﮐﺮوي اﯾﻦ ﻧﺎﻧﻮ ذرات ﺗﻮﺳﻂ دﺳﺘﮕﺎه ﻣﯿﮑﺮوﺳﮑﻮپ اﻟﮑﺘﺮوﻧﯽ روﺑﺸﯽ و ﻋﺒﻮري ﻣﺸﺨﺺ ﺷﺪ. ﻫﻤﭽﻨﯿﻦ اﺛﺮ ﺿﺪ ﻣﯿﮑﺮوﺑﯽ ﻓﯿﻠﻢ ﻫﺎي ﻧﺎﻧﻮ ﮐﺎﻣﭙﻮزﯾﺘﯽ ﺑﺮ ﭘﺎﯾﻪ ﻧﺸﺎﺳﺘﻪ، ﻫﻤﺮاه ﺑﺎ اﯾﻦ ﻧﺎﻧﻮ ذرات ﺑﻪ وﺳﯿﻠﻪ آزﻣﻮن ﻧﻔﻮذ دﯾﺴﮏ و ﺗﻌﯿﯿﻦ ﺣﺪاﻗﻞ ﻏﻠﻈﺖ ﻣﻬﺎرﮐﻨﻨﺪﮔﯽ ﻧﺎﻧﻮ ذرات ﺗﻮﺳﻂ ﺳﻪ ﺑﺎﮐﺘﺮي اﺷﺮﯾﺸﯿﺎ ﮐﻠﯽ، اﺳﺘﺎﻓﯿﻠﻮﮐﻮﮐﻮس اورﺋﻮس و ﻟﯿﺴﺘﺮﯾﺎ ﻣﻮﻧﻮﺳﯿﺘﻮژﻧﺰ ﻣﻮرد ﺑﺮرﺳﯽ ﻗﺮار ﮔﺮﻓﺖ. ﻧﺘﺎﯾﺞ ﻧﺸﺎن داد ﻧﺎﻧﻮذرات ﻧﻘﺮه ﺑﯿﺸﺘﺮﯾﻦ ﺟﺬب را در430 ﻧﺎﻧﻮﻣﺘﺮ ﻧﺸﺎن دادﻧﺪ و داراي ﺷﮑﻞ ﮐﺮوي و ﻣﯿﺎﻧﮕﯿﻦ اﻧﺪازه آﻧﻬﺎ ﺑﯿﻦ 15 ﺗﺎ 37nm ﺑﻮدﻧﺪ. ﻧﺎﻧﻮ ذره ﻧﻘﺮه ﺗﻮﻟﯿﺪ ﺷﺪه ﺑﻪ ﻋﻨﻮان ﻣﻬﺎرﮐﻨﻨﺪه رادﯾﮑﺎل DPPH(IC50 =170 μ M) ﻋﻤﻞ ﮐﺮدﻧﺪ. ﺣﺪاﻗﻞ ﻏﻠﻈﺖ ﻣﻬﺎري رﺷﺪ و ﺣﺪاﻗﻞ ﻏﻠﻈﺖ ﮐﺸﻨﺪﮔﯽ ﺑﺮ روي ﺑﺎﮐﺘﺮي اﺳﺘﺎﻓﯿﻠﻮﮐﻮﮐﻮس اورﺋﻮس 3/1 و 6/2 ﺑﺎﻻﺗﺮﯾﻦ ﻣﯿﺰان ﺣﺪاﻗﻞ ﻏﻠﻈﺖ ﻣﻬﺎر ﮐﻨﻨﺪﮔﯽ را ﻧﺸﺎن داد. ﻫﻤﭽﻨﯿﻦ ﺑﺎ اﻧﺪازه ﮔﯿﺮي ﻗﻄﺮ ﻧﺎﺣﯿﻪ روﺷﻦ ﺑﻮﺳﯿﻠﻪ آزﻣﻮن ﻧﻔﻮذ دﯾﺴﮏ ﻧﺸﺎﺳﺘﻪ ﺧﺎﻟﺺ ﺧﺎﺻﯿﺖ ﺿﺪﻣﯿﮑﺮوﺑﯽ ﻧﺪارد وﻟﯽ ﺑﺎ اﻓﺰودن 250ppm ﻧﺎﻧﻮ ذره ﻧﻘﺮه ﺑﻪ ﻓﯿﻠﻢ ﻧﺸﺎﺳﺘﻪ در ﺣﻀﻮر ﺑﺎﮐﺘﺮي ﻫﺎﻟﻪاي ﺑﺎ ﻗﻄﺮ cm 2/05 ﺗﺸﮑﯿﻞ ﻣﯿﺸﻮد و در واﻗﻊ ﺑﺎ اﻓﺰودن ﻧﺎﻧﻮ ذره ﺧﺎﺻﯿﺖ ﺿﺪ ﻣﯿﮑﺮوﺑﯽ در ﻓﯿﻠﻢ ﻧﺸﺎﺳﺘﻪ ﺷﮑﻞ ﻣﯿﮕﯿﺮد و ﺑﺎز ﺑﺎ رﺳﺎﻧﺪن ﻣﻘﺪار ﻧﺎﻧﻮ ذره ﻧﻘﺮه ﺑﻪ500ppm در ﻓﯿﻠﻢ ﻗﻄﺮ ﻫﺎﻟﻪ ﺑﻪ 2/35cm اﻓﺰاﯾﺶ ﻣﯿﯿﺎﺑﺪ ﮐﻪ ﻧﺸﺎن دﻫﻨﺪه اﻓﺰاﯾﺶ ﺧﺎﺻﯿﺖ ﺿﺪ ﻣﯿﮑﺮوﺑﯽ اﺳﺖ. ﻓﯿﻠﻢ ﻫﺎي زﯾﺴﺖ ﺗﺨﺮﯾﺐ ﭘﺬﯾﺮ ﺑﺮ ﭘﺎﯾﻪ ﻧﺸﺎﺳﺘﻪ ﻫﻤﺮاه ﺑﺎ ﻧﺎﻧﻮ ذرات ﻧﻘﺮه ﺑﯿﻮﺳﻨﺘﺰ ﺷﺪه اﺛﺮ ﺿﺪﻣﯿﮑﺮوﺑﯽ ﻋﻠﯿﻪ ﻫﻤﻪ ﻣﯿﮑﺮوب ﻫﺎي آزﻣﻮن ﺷﺪه ﻧﺸﺎن داد )0/05

Abstract Today, due to its environmental problems and importance, the use of biodegradable nanocomposite films is of particular importance. In this study, silver nanoparticles were synthesized by means of aqueous extract of Mentha and the adsorption capacity of 1,1-diphenyl-2-picrylhydrazyl radicals and their antimicrobial activity should investigated by the method of determination of minimum inhibitory concentration. The uniformity and shape were structure of these nanoparticles was determined by scanning electron microscopy. The antimicrobial effect of starch-based nanocomposite films, together with these nanoparticles, was investigated by disk diffusion test and determination of minimum inhibitory concentration of nanoparticles by three Escherichia coli, Staphylococcus aureus and Listeria monocytogenes. The results showed that silver nanoparticles showed the highest absorption at 430 nm and had a spherical shape and their average size was between 15-37 nm. The silver nanoparticles produced acted as DPPH radical inhibitors (IC50 = 170 μ M). The minimum growth inhibitory concentration and the minimum lethal concentration on Staphylococcus aureus showed 3.1 and 6.2 showed the highest minimum inhibitory concentration. Also, by measuring the diameter of the bright area by the disk penetration test, pure starch does not have antimicrobial properties, but by adding 250 ppm silver nanoparticles to the starch film in the presence of bacteria, a halo with a diameter of 2.55 cm is formed and in fact by adding nanoparticles antimicrobial properties Starch is formed in the film and again increases the diameter of the halo to 2.35 cm in the film by bringing the amount of silver nanoparticles to 500 ppm, which indicates an increase in antimicrobial properties. Starch-based biodegradable films with biosynthesized silver nanoparticles showed antimicrobial effect against all tested microbes (p <0.05).