تشخيص سوياي تراريخته از طريق ژن‌‌هاي Camv 35S Promotor و Lectin در نمونه‌هاي غذاي كودك

Detection of GMO soybean by Camv 35S Promoter and Lectin genes in baby food and soybean samples

اﻣﺮوزه ﺑﻪ دﻟﯿﻞ اﻓﺰاﯾﺶ روزاﻓﺰون ﺟﻤﻌﯿﺖ، ﺳﻄﺢ زﯾﺮ ﮐﺸﺖ ﻣﺤﺼﻮﻻت ﺗﻐﯿﯿﺮ ﯾﺎﻓﺘﻪ ژﻧﺘﯿﮑﯽ )(GMO در ﺗﻤﺎﻣﯽ ﮐﺸﻮرﻫﺎ اﻓﺰاﯾﺶ ﯾﺎﻓﺘﻪ اﺳﺖ. در اﯾﺮان ﺳﯿﺴﺘﻤﯽ ﺑﺮاي ﺑﺮﭼﺴﺐ ﮔﺬاري ﺑﺮ روي ﻣﺤﺼﻮﻻت ﺗﺮارﯾﺨﺘﻪ وﺟﻮد ﻧﺪارد. ﻏﺬاي ﮐﻮدك ﮐﻪ ﯾﮑﯽ از ﻣﻬﻢ ﺗﺮﯾﻦ ارﮐﺎن رﺷﺪ و ﺗﻀﻤﯿﻦ ﮐﻨﻨﺪه ﺳﻼﻣﺖ ﮐﻮدك اﺳﺖ، از اﯾﻦ ﻗﺎﻋﺪه ﻣﺴﺘﺜﻨﯽ ﻧﯿﺴﺖ. از اﯾﻦ رو، در اﯾﻦ ﭘﮋوﻫﺶ ﺳﻌﯽ ﺷﺪ ﮐﻪ ﺑﻪ ﮐﻤﮏ ﺑﺮرﺳﯽ ژن ﮐﻨﺘﺮل داﺧﻠﯽLectin و ژن ﺷﺎﺧﺺ ﺗﺮارﯾﺨﺘﮕﯽ Camv 35S Promoter، ﻣﺤﺼﻮﻻت ﺗﺮارﯾﺨﺘﻪ ﺑﺎ روش واﮐﻨﺶ زﻧﺠﯿﺮه اي ﭘﻠﯽ ﻣﺮاز(PCR) ﺷﻨﺎﺳﺎﯾﯽ ﺷﻮﻧﺪ. ﺑﺮاي اﯾﻦ ﻣﻨﻈﻮر 25 ﻧﻤﻮﻧﻪ ﺳﻮﯾﺎ از ﻣﻐﺎزه ﻫﺎ و 25 ﻧﻤﻮﻧﻪ ﻏﺬاي ﮐﻮدك از داروﺧﺎﻧﻪ ﻫﺎي ﺷﻬﺮ ﺗﻬﺮان و ﯾﮏ ﻏﺬاي ﮐﻮدك ﺧﺎرﺟﯽ ﺑﺎ ﺑﺮﭼﺴﺐ ﺗﺮارﯾﺨﺘﮕﯽ ﺧﺮﯾﺪاري ﺷﺪ. DNA ﻧﻤﻮﻧﻪ ﻫﺎ ﺑﺎ ﮐﯿﺖ اﺳﺘﺨﺮاج ﺑﺎ ﺑﺮﻧﺪ TLABاﺳﺘﺨﺮاج ﺷﺪ. ﭘﺲ از ﺑﺮرﺳﯽ ﻏﻠﻈﺖ و ﮐﯿﻔﯿﺖ DNA اﺳﺘﺨﺮاج ﺷﺪه ﺗﻮﺳﻂ دﺳﺘﮕﺎه ﻧﺎﻧﻮدراپ، ﻧﻤﻮﻧﻪ ﻫﺎ ﺑﻪ وﺳﯿﻠﻪ روش PCR ﻣﻮرد ﻏﺮﺑﺎﻟﮕﺮي ﻗﺮار ﮔﺮﻓﺘﻨﺪ. ﻧﺘﺎﯾﺞ ﺑﺮرﺳﯽ ﻧﻤﻮﻧﻪ ﻫﺎي ﺳﻮﯾﺎ ﻧﺸﺎن داد ﮐﻪ، 92% از ﻧﻤﻮﻧﻪ ﻫﺎ داراي ژن داﺧﻠﯽLectin و 63% داراي ژن Camv 35S Promoter ﺑﻮدﻧﺪ. از 25 ﻧﻤﻮﻧﻪ ﻏﺬاي ﮐﻮدك 93% ژن داﺧﻠﯽLectin و 52% ژن ﺷﺎﺧﺺ ﺗﺮارﯾﺨﺘﮕﯽ Camv 35S Promoter را داﺷﺘﻨﺪ. وﺟﻮد ژن Camv 35S Promoter ﺑﯿﺎﻧﮕﺮ ﺗﺮارﯾﺨﺘﻪ ﺑﻮدن ﻣﺤﺼﻮل اﺳﺖ. ﻣﯽ ﺗﻮان ﻧﺘﯿﺠﻪ ﮔﺮﻓﺖ ﮐﻪ در ﻧﻤﻮﻧﻪ ﻫﺎي ﺳﻮﯾﺎ ﻣﯿﺰان ﺗﺮارﯾﺨﺘﮕﯽ ﺑﻪ ﺻﻮرت ﻗﺎﺑﻞ ﺗﻮﺟﻬﯽ زﯾﺎد اﺳﺖ و در ﻏﺬاي ﮐﻮدك ﻣﯿﺰان ﺗﺮارﯾﺨﺘﮕﯽ در ﻧﻤﻮﻧﻪ ﻫﺎي اﯾﺮاﻧﯽ ﺑﯿﺸﺘﺮ از ﻧﻤﻮﻧﻪ ﺧﺎرﺟﯽ ﻣﯽ ﺑﺎﺷﺪ. در ﺣﺎﻟﯽ ﮐﻪ ﻫﯿﭻ ﯾﮏ از ﻧﻤﻮﻧﻪ ﻫﺎي ﻣﻮرد ﺑﺮرﺳﯽ در اﯾﻦ ﺗﺤﻘﯿﻖ ﺑﺮﭼﺴﺐ ﺗﺮارﯾﺨﺘﮕﯽ ﻧﺪاﺷﺘﻨﺪ.

In most countries today the area under cultivation of transgenic crops has increased due to the increasing population.In Iran, there is no system for labeling transgenic products.Although baby food is one of the most important needs for growth and health of the baby, it is no exception.Therefore, in this study,we try to identify the transgenic products by PCR by examining the internal control gene Lectin and Camv 35S Promoter gene transcriptome.25 soybean samples were collected from shops and Twenty-five samples of baby food were collected from pharmacies in Tehran. Samples were extracted with TLAB brand tissue extraction kit. After analyzing the concentration and quality of DNA extracted by the nanodrop machine, the samples were screened by PCR.25 soybean samples were analyzed for 23 of the total samples (%92) for the presence of Lectin internal gene and 15 samples from all samples (%63) had Camv 35S Promoter gene.Of the 25 samples of baby food, 20 (%83) were positive for the presence of the inner gene,Lectin positive, and 13 (%52) were positive for Camv 35S Promoter gene transformation index. None of the products containing Camv 35S Promoter gene, indicating that the product was transgenic, were not labeled.It can be concluded that the rate of transgenicity in the samples tested is significantly higher.In baby foods, the transgenicity rate of Camv 35S Promoter gene was higher in Iranian samples than in foreign samples. In soybean samples, Camv 35S Promoter gene was present in Iranian products, while none of the samples tested had transgenic label