ارزيابي درمان تركيبي سلول هاي بنيادي مشتق از مغز استخوان (BM-MSCs) و لوسيون ترميم كننده حاوي عسل، پروپوليس و آلوئه ورا در بهبود زخم سوختگي درجه دو در مدل حيواني

Evaluation of the combined treatment of bone marrow-derived stem cells (BM-MSCs) and restorative lotions containing honey, propolis and aloe vera in the healing of second-degree burn wounds in the animal model

يكي از چالش هاي علم پزشكي ترميم زخم هاي سوختگي است. هدف از مطالعه حاضر ارزيابي درمان با لوسيون حاوي عسل، آلويه ورا، بره موم و سلول هاي بنيادي مشتق از مغز استخوان در بهبود زخم سوختگي درجه دو در مدل حيواني است. روش بررسي اين مطالعه تجربي در حيوان خانه دانشكده پزشكي دانشگاه علوم پزشكي تهران، از مهر ماه 1399 تا آبان ماه 1399 انجام شد و 72 رت پس از سوختگي در شش گروه با دوره هاي 7، 14 و 21 روزه تقسيم بندي شدند: شاهد (بدون درمان)، كنترل مثبت (پماد سيلورسولفاديازين 1%)، تجربي اول (سلول هاي بنيادي)، تجربي دوم (لوسيون)، تجربي سوم (سلول هاي بنيادي و لوسيون)، تجربي چهارم (DMEM). از سطح زخم در روزهاي صفر، 4، 7، 10، 14، 17 و 21 عكس تهيه شد و مساحت زخم توسط نرم افزار J image محاسبه گرديد. در روزهاي 7، 14، 21 نمونه ها جهت رنگ آميزي H&E، تري كروم ماسون و بررسي هاي IHC و RT-PCR تهيه شدند و نتايج با نرم افزارGraph Pad Prism8 و آزمون هاي Analysis of variance، Two Way و Tukeyبررسي شد. يافته ها ارجحيت درمان با گروه درمان تركيبي و سپس با گروه لوسيون بود. در تصاوير ماكروسكوپيك و ميكروسكوپيك، بيشترين ميزان پوشش زخم و حضور رشته هاي كلاژن بالغ در گروه درمان تركيبي مشاهده شد. نتايج آناليزها نيز بيانگر افزايش اپيتليزاسيون، گرانوليشن، رسوب كلاژن، آنژيوژنز و تكثير سلول هاي فيبروبلاست و كاهش التهاب در گروه درمان تركيبي بود. گروه درمان تركيبي بيشترين ميزان بيان ژن TGF-β را دارا بود (05/0

One of the most important issues in medical science is the healing of burn wounds. The use of medicinal plants has been common for many years and today cell therapy offers new approaches to the management of skin wound healing. The present study aimed to evaluate the the combination treatment of lotion containing honey, Aloe, and propolis with BM-MSCs in the healing of second-degree burns in animal models. Methods: this experimental study from October 2020 to November 2020, was performed in the animal house of Tehran University of Medical Sciences. 72 rats with an age range of 3-4 months and a weight of about 200-250 gr, after burns were randomly divided into 6 groups of 12 with study periods of 7, 14 and 21 days. The groups were as following: control (no treatment), Positive control (SSD1%), First experimental (Stem cells), Second experimental(lotion), Third experimental(Stem cells and lotion), and Fourth experimental (DMEM). To measure the percentage of healing from the wound surface on days 0, 4, 7, 10, 14, 17, and 21 photos were taken and the wound surface area was calculated by Image J software. After sacrificing rats, tissue samples were taken on days 7, 14 and 21 after burning induction. Samples were prepared for staining H&E and Trichrome Masson’s, as well as RT-PCR examination. The results were analyzed using Graph Pad Prism8 software and Tukey and one-way variance tests. Results: Treatment preference was with the combination therapy group and then with the lotion group. According to macroscopic and microscopic images of H&E and Trichrome Mason, the highest amount of wound coverage and the presence of mature collagen fibers were also observed in the combination therapy group. The results of statistical analysis and scoring also showed increased epithelization, granulation tissue formation, collagen deposition, angiogenesis and fibroblast cell proliferation, and decreased inflammation in the combination therapy group. The combination therapy group also had the highest expression of the TGF-β gene. (P<0/05) Conclusion: Due to its epithelialization, anti-inflammatory and angiogenesis properties, the lotion has healing properties in second-degree burn wounds and its simultaneous use with mesenchymal stem cells leads to healing and acceleration of burn wound healing.