آزمايشگاهي SCLEROTINIA SCLEROTIORUM روش هاي توليد اسكلروت و آپوتسيوم از قارچ در شرايط

Production Methods of Sclerotia and Apotbecium from Sclerotinia sclerotiorum in the Laboratory Conditions

In this study, different methods for rapid production of apothecia and ascospores that play important role in pathogenicity tests and species identification, were evaluated . In order to obtain the most and biggest sclerotia, four different methods were examined. Among them, the method used by Kohn and Sunsford gave the best results. Sclerotia from sunflower, rapeseed, cheiranthus and lettuce in this method were kept in refrigerator (4°C) for three months. To produce apothecia by the method of Paterson and Gerogan, sclerotia were transferred to Petri plates containing 1% WA and pots contaning sterilized vermiculate and were kept at 10°C and 3 5°C with 16/8 hour light/dark cycle, respectively. After 4-6 weeks in vermiculate and 5-7 weeks in WA, complete apothecia were frequently produced in great numbers. On the basis of these results and considering that under natural conditions more than 8 months is needed for apothecium production, this method is proposed for obtaining enough accosporic inoculum for laboratory and greenhous studies.