كليدواژه :
كلزا , محيط كشت , Callus formation , explant , genotype , MEDIA , Rapeseed , ريزنمونه , ژنوتيپ , كشاورزي
چكيده لاتين :
This experiment was conducted to study the effect of genotype, explant and culture media on callus induction and growth in media with and without growth regulators. Nine pure lines of rapeseed, Orakel, ACSN1, PF504588, PF7O4591, Bounty, Lisandra, Shiralee, Maluka and Global grown in five MS media supplemented with different combination of hormones as control(0 BAP, O 2,4-D),A(l BAP, 0.5 2.4-D),B(2BAP, 1 2,4-D),C(3 BAP, 1.5 2,4-D) and D(1 BAP, 2 2,4-D) and four type of explants as hypocotyl, cotyledon, seed and root were used. Ten explants of hypocotyl (O.9-1 cm), and cotyledon with 2mm petiole, five explants of root (0.9-1 cm) and 1O seeds were used in each experimental unit. The experiment was factorial based on completely randomized design with four replications. After one month, percent of callus initiation, callus diameter, callus fresh weight, callus dry weight and water content of callus were determined. Because of differences between control media and the media having growth regulators, analysis of data from two media was done separately with SAS program. Comparison of means in growth regulator media showed that, in all traits, effect of genotype, media and explant were significant (p=l %) except for percent of callus formation in different media. First order interaction and second order interaction were significant in all traits except dry weight of callus. Between all genotypes, Bounty and Global showed the highest callus growth and Maluka and Orakel the lowest in growth regulator media. On the whole, medium A was the best media and seed was the best explant in this medium. Statistical analysis in the media without growth regulator showed that the effect of genotype, explant and interaction between them was significant. Maluka and Orakel had the highest callus formation in the media without growth regulator, indicating that endogenous hormones were higher in these lines, and these hormones probably inhibit the callus formation in the media.
