آر براي رديابي آنها RT-PCR گروه بندي سرولوژيكي جدايه هاي ويروس موزاييك خيار در كدوييان مناطق باسمنج و شبستر، و كاربرد

Serological Sub grouping of Basmenj and Shabestar Isolates of Cucumber Mosaic Virus and Application of RT-PCR for the Virus detection

Cucurbits are widely grown in East Azarbaijan. Since cucumber mosaic cucumovirus (CMV) infects cucurbits worldwide, infection of cucurbits by CMV in East Azarbaijan was surveyed. The plants showing suspected symptoms were collected and subjected to enzyme-linked immunosorbent assay (ELISA) with CMV- and or CMV subgroup I- specific antibodies. Then, total RNA was extracted from each sample by a quick and simple phenol and chloroform extraction protocol. The total RNAs were subjected to an RT-PCR assay with a primer pair specific to coat protein coding region of the virus. An expected approximately 870 bp DNA fragment was amplified from many samples. These results showed a high incidence of the virus in cucurbits of Azarbaijan and revealed that CMV subgroup I isolates were involved in the infection.