U921 ژن PCR-RFLP از طريق KOS جداشده از بيمار ايراني با سويه )HSV-1( مقايسه مولكولي ژنوم ويروس هرپس سيمپلكس نوع 1

Molecular Comparison of Herpes Simplex Viruse Type 1 (HSV-1)Genomes Isolated from an Iranian Patient with KOS Strain via the amplification of UL29 gene (DNA Binding Protein)

Herpes Viruses genome contains a linear DNA and is a good model for eukaryotic DNA replication system. The restriction map of different HSV-1 strains has been identified in the developed countries .In our country; Iran, the HSV-1 viruses isolated have been detected and isolated from patients using immunological techniques, but molecular genetic aspects need to be studied more in the future. In this study viruses were amplified using Vero cells. It was demonstrated that this isolate was HSV-1. This verification was done using southern blotting in which the probe was prepared from UL29 gene cloned in PNN-1 plasmid. In addition, it was shown that HSV-1 isolated from the Iranian patient was identical with HSV-1 KOS strain . The amplification of UL29 gene via PCR-RFLP was performed with 5 restriction enzymes. In summary, this study proved that other isolates can also be used in molecular epidemiological studies of different Iranian populations.