ART بررسي تاثير فلور ميكروبي سرويكس بر روي نتايج باروري حاصل از سيكل هاي

THE EFFECT OF CERVICAL MICROBIAL FLORA ON FERTILITY RATE IN A. R. T CYCLES

Introduction: One of the most important steps in ART cycle is Embryo transfer followed by the sensitive stage of implantation, which depends on two main factors; embryo quality and receptivity of Endometrium . Another important factor in endometrial reception is existence of microorganisms in cervical canal which can involve normal flora of Endometrium. They can have a harmful effect on implantation process by activating the immunity system upon entering the uterus. Materials & Methods: This descriptive and analytic study was done on 90 infertile patients aged between 20-40 years. Ovulation induction protocol was the same in all patients. In ovarian puncture, the first specimen was taken from the cervical canal before vaginal washing for microbial culture, while the second specimen was taken from cervical canal before any washing on transfer day after 48-72hrs. The third specimen was from about 1.5-2 cm of transferred catheter that was cut and put in culture media. All of the specimens were cultured and evaluated for presence of gram positive and negative bacteria. Results: ertility rate in the two groups; the group whose culture specimen was sterile (31pt ) and the one whose culture specimen was infected ( 49 patients) were not significant statistically. The group with a sterile catheter tip (29pts) was compared with the group with infected catheter tip ( 51pts). Fertility rate was 6.9% in the infected catheter group and 19.6% in the sterile catheter group. Although fertility rate in sterile group was 2.8 times more than in the infected group, it was not statistically significant. Frequency of bacteria didn,t effect the fertility rate. Fertility rate in the gram pos. group in comparsion with the gram neg. group was 2.83 times, but not significant in the statistical analysis. Conclusion: In our study, infection of catheter tip with gram neg. bacteria had no statistically significant effect on fertility rate, but it still counts as a main factor for successful IVF/ ET results.