چكيده لاتين :
To improve the fertility potential of infertile women, gametes, embryo and ovarian tissue are preserved using cryopreservation. Ovarian cryopreservation has more advantages as compared to oocyte freezing. Ovarian tissue has a lot of follicles which can be used in sequential ovarian cycles. Ovarian preservation is useful for treatment of infertile patients undergoing chemotherapy and radiotherapy. The first reports about cryopreservation of ovarian tissue were published in 1950s and after that, human ovarian freezing was reported in 1995. There are three methods for freezing ovaries; slow freezing methods, vitrification and ultrarapid. In these techniques, the ice formation is inhibited for enhancing the viability of the cells after freezing. The main factors for freezing of tissue are cryoprotectants, velocity of cooling and freezing rate which are dependent on the kind of the cell and tissue, permeability of the membrane and the ratio of the surface and the volume of the cell. Transplantation and invitro development are used for development of cryopreserved ovarian follicles.
