تهيه آنتي ژن حام مسموماتيك از آسپرژيلوس فوميگاتوس براي تشخيص

PREPARATION OF SOMATIC ANTIGEN FROM ASPERGILLUS FUMIGATUS FOR SERODIAGNOSIS OF ASPERGILOSISS.

Aspergilosis is a mycotic infection caused by various species of the genus aspergillus . This disease shows different clinical forms in regard to predisposing Factors and pathegenesis of the disease . Clinical and mycological diagnosis of the disease is unspecific and problematic , but serology can be helpful for diagnosis and for following up the treatment . The aim of this study is preparaytion of somatic antigen and related antiserum in Iran to perform precipitation tests for diagnosing the noninvasive froms of aspergilosis . This can cause needlessness from foreign products . Aspergilus fumigatus was cultured in glucose - peptone broth and shaken in 30" c for 3 days. The harvested colonies were homogenized by seasand in mortar and the cell extracts were purified concentrated and injected subcutaneously in rabbits for producing hyperimmune antiserume:. Antigens and antisera was evaluated by counterimmunoelectro phoresis test . The prepared antigens had enough antigenesity for producing high titer of antibody in the rabbits . It also reacted with rabbit and human patientsʹ serum . The number and pattern of the precipitation lines were similar and comparable with standard antigen and antiserum .