تاثير فاكتورهاي مهاركننده لوسمي انساني بر تعداد سلول هاي توده اي داخلي بلاستوسيست هاي موش سفيد آزمايشگاهي در محيط آزمايشگاه

The Effect of the Recombinant Human Leukemia Inhibitory Factor (RLIF) on the Number of Blastocyst Inner-Cells Obtained from Mouse Two-Cell Embryos in vitro

Introduction: This study was performed to investigate the rate of inner cell mass of blastocyst which was obtained from the two-cell embryos of mouse culture in the presence and absence of recombinant of human leukemia inhibitory factor. Materials and Methods: ICR female mice (6-8 weeks old) received intra peritoneal injection of 7.5 IU of pregnant mare serum gonadotropine for super ovulation. This was followed by intra peritoneal administration of 7.5 of hCG 46-48 hours later. The mice were then mated to mature ICR male mice and were checked for vaginal plug 20 hours later. Female mice were killed by cervical dislocation 48-50 hours after hCG administration. After washing flushing of the oviduct from the proximal end of the oviduct, two cell embryos were selected and collected. All two cell embryos were randomly divided into 4 groups (A, B C and D) and were cultured in special media for 120 hours in CO2 incubator. Group A: KSOM+AA, Group B: KSOM+AA 500 IU/ml LIF. Group C: KSOM+AA 1000 IU/m1 LIF. Group D: KSOM+AA 1500 IU/ml LIF media and were cultured in special media for 120 hours in CO2 incubator. Later, all blastocysts were collected and ICM was counted by differential staining technology. Results: The rates of ICM of blastocysts, which were obtained from different groups, were 19±2.6, 28±4.4, 24±2.1, and 26±2.2, respectively. This data indicated that the rate of ICM in B, C and D was statistically higher than A (p=0.02) Moreover, there was no statistical difference between the three groups (B, C and D). Conclusion: In brief, since leukemia inhibitory factor can improve the rate of ICM of blastocyst, it is suggested that this factor be added to blastocyst culture medium.