بررسي نوع و فعاليت آنزيم هاي موجود در موارد دفعي -ترشحي پروماستيگوت ليشمانياماژور

Evaluation of Deferent Enzymes Activity in Excretory and Secretory (EIS) Products of Leishmania major Promastigotes

Cutaneous leishmaniasis is a common disease in the world, which is caused by Lishmania protozoa. Protective procedures, such as vaccination were not successful so far. Leishmania parasites secret some proteinous products which some of them has enzymatic activity. These enzymes could play a role in some biological function in parasites life cycle. Aims of this study were to detect proteins excreted/secreted in parasite culture. The promastigote forms of the parasite were cultured in RPMI 1640 containing 10% FCS and antibiotics at 22°C. Forth passage parasites were transferred to RPMI without FCS and antibiotics. After 0, 6, 12, 24, 48 and 72 hours, using centrifugation and filters of 0.22µ the EIS products was separated. Activity of Acid Phosphatase (ACP) and Acetylcholinesterase (AChE) were detected and measured in E IS products. Activity of AChE was negative at zero time, but increased after 6 hrs and reached plateau after 24 hrs, and then decreased after this time. Activity of ACP at zero time was very low but reached to maximum levels at 6 hrs and declined after wards. Using SDSPAGE three protein bands were seen, which corresponded to and 130, 110 and 75 KD. Probably these bonds are related to ACP. We hope these EIS enzymes could play some role in future vaccination and immunization of cutaneous leishmaniasis.