L.L MAJOR آلوده به BALB/C به بهبود زخم جلوگيري از گسترش ليشمانيوز در موش ALHANNA TINCTORIA و EUPHORBIA MYSINITES, PEGANUM HAYMALA بررسي آثار فرمولاسيون گياهي جديد از

Effects of New Herbal Formulation of Euphorbia Mysinites, Alkanna Tinctoria and Peganum Harmala Combination Against Experimental Leishmaniasis in Balb/c Mice

Introduction: The aim of the present study is to find the effect of new herbal formulation of Euphorbia mysinites, Alkanna tinctoria and Peganum harmala combination on experimental Leishmaniasis in Balblc mice. Material & Methods: To carry out this investigation, 6 groups of Balblc mice (each with 8 mice) were selected as healthy negative control group, non-treated control group, group treated with glucantime, group treated with herbal drugs orally, group treated with herbal ointment and group treated with herbal ointment and syrup. All groups except the healthy negative control group were inoculated experimentally with 2x106 promastigotes by injection of the parasites in their tail. Diameter of the ulcers were measured weekly by caliper for 4 months. At the end of fourth month, all mice were killed and their spleen were investigated for the presence of the parasite. Results: The results indicated that Peganum harmala, Alkanna tinctoria, Euphorbia mysinites can affect sore and visceral forms of Leishmaniasis in the Balblc mice. The death rates and relapse of infection in group treated with the herbal combination were less than other groups. In addition, in groups treated with glucantime, 25% of the mice were cured completely and 37.5% showed relapsing. Meanwhile in group treated with herbal combination orally, 50% of the mice were cured completely and 37.5 % showed relapsing. Moreover, in group treated with herbal ointment, 37.5 % of the mice were cured completely and 50% showed relapsing and in group treated with herbal ointment and syrup, 25 % of the mice were cured completely and 37.5% showed relapsing. During the experiment no side effect due to the herbal combination was observed in the treated mice. Conclusion: The present herbal combination in comparison with glucantime is more effective on murine Leishmaniasis, hence it could be suggested as a substitute for glucantime in the treatment of leishmaniasis.