مقايسه تركيبات استروييدي و غيراستروييدي از حيث سيتوتدكسيسيته و مهار فعاليت ماتريكس متالوپروتيينازها در مدل سلولي

Comparison of Cytotoxcity and Inhibitory Effect of Steroidal and Non- Steroidal Anti-inflammatory Drugs on Matrix Metalloproteinase Activity in Cell Culture Model

Matrix metalloproteinaseʹs (MMPs) are critical enzymes in some pathological stages such as Rheumatoid arthritis and cancers. We studied the effect of two Non-Steroidal Anti-Inflammatory Drugs (Diclofenac and Piroxicam) and a synthetic glucocorticoid, Dexamethasone in an In vitro cell cultured model using cytotoxcity analysis and zymography assay. Based on our data, all above mentioned drugs are able to induce Inhibitory effect on Matrix Metalloprotelnase (MMPs) expression. The comparison of Inhibitory potency of these agents in a concentration range between 10 to 200 g/mL revealed that Diclofenac has more cytotoxic effect than the others. Low dose administration of Diclofenac (10-20 Og/mL), is applicable for relieving the patients problem, whereas at high dose It induces cytotoxic effect on cell proliferation and cause cell death. High doses administration of Piroxicam and Dexamethasone show little cytotoxcity on cell culture. Furthermore, the cytotoxic effects of these agents are concomitant with their effect on MMPs expression.