تاثير ميدان هاي الكترومغناطيس نوسان دار با فركانس بسيار پايين در ترميم زخم پوستي موش هاي صحرايي ديابتي

Effect of Extremely Low Frequency Pulsed Electromagnetic Fields on Wound Healing in the Skin of Diabetic Rats

Delayed wound healing is a common complication in diabetes mellitus and the using of Extremely Low Frequency Pulsed Electromagnetic Fields (ELF PEMF) has been shown its effectiveness in reducing the wound healing duration. From this point of view, the main purpose of present study is to investigate the effect of Extremely Low Frequency Pulsed Electromagnetic Fields (ELF PEMF) on skin wound healing in diabetic rats. In this study diabetes was induced in male wistar rats via single subcutaneous injection of 65-mg/kg streptozocin (freshly dissolved in sterile saline, 0.9 %). One month after the induction of diabetes, a full-thickness dermal incision (35 mm length) was made on the right side of the Para vertebral region. The wound was exposed to ELF PEMF (20 Hz, 4 ms, 8 mT) for 1 hour daily. Wound healing was evaluated by measuring surface area, percentage of healing, and duration of healing and wound tensile strength. Obtained results showed that the duration of wound healing in diabetic rats in comparison with the control group was significantly increased. In contrast, the rate of healing in diabetic rats receiving PEMF was significantly greater than diabetic control group. The wound tensile strength also was significantly greater than the control animals. Besides, the duration of wound healing in control group receiving PEMF was less than the sham group. Based on the above-mentioned results we concluded that, this study provides some evidence to support the use ELF pulsed electromagnetic fields to accelerate diabetic wound healing. Further researches are needed to determine the PEMF mechanisms in acceleration of wound healing in diabetic rats.