بررسي اثر كافيين بر القاي آپوپتوزيس در سلول هاي منوسيت خون

Study of the Effect of Caffeine on Induction of Apoptosis in Blood Monocyte Cells

Caffeine is one of the active alkaloids has been used in the treatment of asthma and apnea in the human newborn. Its pharmacological activity varies depending on the dose and the type of target cells. In this study, the effect of caffeine on induction of apoptosis in blood monocyte cells was investigated. In this study, human blood monocytes from the peripheral blood were prepared and the effect of various concentrations of caffeine on survival rate, super oxide anion production and DNA fragmentation was investigated. The results show that caffeine effects are highly dose dependent. Low concentrations (<10 mM), enhance cell survival with a peak at 5 mM. At these concentrations, caffeine failed to affect super oxide anion production and DNA fragmentation. At moderate concentrations of caffeine (5-20 mM), the viability reached to the control and at higher concentrations of caffeine (>20 mM) inhibited cell survival and the 02 production was increased, while DNA fragmentation was similar to the control. The results suggest that low concentrations of caffeine prevent apoptosis of monocyte cells, whereas at moderate concentrations of caffeine induces apoptosis.