در روند تكامل غضروف و پيدايش مراكز استخوانسازي دنده ها در جنين رت GaL Nac مطالعه نقش قند انتهايي

Study on role of Gal Nac terminal sugar on development of cartilage and ossification centers of ribs in rat

Terminal sugras play an important role in development, differentiation, migration , exchange and communication of mature cells. Manner of terminal sugar determines specialization of the cell and the way that it responses to hormones. Development of ribs requires reciprocal interactions between sclerotome portion of paraxial mesoderm and structures around it Terminal sugars and extra cellular matrix play an important role in regulation of these interactions. The aim of present study was to investigate the presence and distribution of cell surface terminal sugars in developing cartilage and ossification centers in ribs of rat embryo 50 Wistar rats chose by simple random sampling. After adaptation and copulation embryos from 8-18 days of gestation were collected. Specimens fixed in B4G and Caronyʹs solutions. After routine histological processing and embedding in paraffin, blocks were cut into 4- micrometer thickness sections. Then sections were stained by histochemical methods (Alcian blue, PAS-Alcian blue) and lectin histochemistty (VVA-B4). Presence of Gal Nac in developing ribs confirmed. This study showed that changes in surface terminal sugars regulate morphologic changes and the fate of the cells during tissue interactions.