بررسي آلودگي ميكروبي بخش هاي مراقبت ويژه بيمارستان هاي همدان

Survey on microbial Contamination of intensive care wards of Hamadan Hospitals

In order to identify major microbial agents that causing nosocomial infections and also detection of drug resistancy to current antibiotics, a cross-sectional study was carried out in two hospitals of medical Sciences University of Hamadan .In this study 620 samples were collected from facilities, devices and different parts of intensive care units (ICU) and coronary care units (CCU) for one year. The samples were also collected from wards air, wards floor, walls, kitchen sink, sewage, hospital beds, staffʹs clothes, gawn.s, staffsʹ hands, suction device, incubator, oxygen mask, ambo hag mask, thermometer, manometer, ventilator, vaso-catheters, stethoscope diaphragm, gavage liquid and electrodes of ECG. The samples were cultured on E.M.B and Blood agar by sterile wet cotten swabs. A smear was also prepared for gram staining. After detection of microbial agents, antibiogram were performed for strains by agar disk diffusion method. The mean of frequency of contamination was 32.7% in two hospitals- The distrihiution of gram negative bacteria was 423% and gram positive bacteria was also 57.7%. The rate of contamination in ICU was 31.5% and in CCU was 33.9% respectively. The most important bacteria were.ʹ Micrococci species, Staph aureus and epidemidis, E-tali, Bacillus .subtilis, Klehsiella, Enterobacter, Pseudomonas, Diphtheroieds and Entrecocci. The most drug resistancy were observed in Staphylococci, Micrococci, klebsiella, Enterococci and Pseudomonas aeroginosa. There was high resistancy to ampicillin, tetracycline, sulfametoxazol and penicillin.