RAPDS ارزيابي تنوع ژنتيكي و گروه بندي نمونه هاي عدس با استفاده از صفات مورفولوژيكي و نشانگرهاي

Evaluation of genetic variation and phylogenetic relationships among lentil accession using morphological trait and RAPD analysis

Lentil (Lens culinaris Medik.) as one of the most important members of the Fabaceas plays fundamental role in the nutrition of the people in developing countries due to its high percentage of protein. Since it is very necessary to determine diversity among the available germplasm, 220 accessions in the seed bank of Agricultural College of Ferdowsi University of Mashhad were studied. The accessions were cultured on the basis of augmented design in fall of 2002. 16 different morphological traits were evaluated among them. On the basis of yield, 64 accessions were assessed for genetic diversity. using Random Amplified Polymorphic DNA (RAPD) analysis was assessed the genetic variation. En this regard, a total of 9 random primers were used to generate RAPD bands determine genetic variation of accession, using genetic distance analysis. Out of the 9 primers, the OPL-19 showed the highest efficiency in discrimination of accession analysis to reveal phylogenetic relationships among the accessions. A wide range based on morphological data were 0.58 - 2.6 and based of molecular data were 0.21 -- 0.96. Based on morphological traits, two genotypic groups were distinguished at %90 similarity level, however, on the basis of molecular data, 11 genotype groups were differentiated based on %90 dissimilarity level. Diversity index of morphological traits was ranged from 0.36 for number of two seeded pods to 0.83 for number of seeds per plant. The index of genetic variation was %80, showing that there are high genetic variation among accessions. Furthermore the data suggested that there was correlation of %83 between RAPD and morphological matrix. So it seems that the RAPD method is an effective tool for genetic analysis of lentil germplasm.