در شرايط طبيعي و تاثير تغيير عناصر و قند بر بيوسنتز اين آلكالوييدها در كشت بافت آن HYOSCYAMUS RETICULATUSL. بررسي تغير ميزان الكالوييدهاي تروپان در مراحل مختلف رشد گياه

Research of change of tropane alkaloids quantities in different stages of growth of Hyoscyamus reticulatus L. in natural condition and ssessment of suger and elements changes on biosynthesis of these alkaloids in In vitro

Hyoscyamus reticudatus from Solanaceae is an important pharmacological plant which contains tropane alkaloids. Presence of tropane alkaloids in this plant is investigated by HPLC apparatus. Different parts of plant including root, stem, leaf, flower and seed in different phases of vegetative growth (two leaves, five leaves, ten leaves), flowering, full flowering and fruiting have been prepared for this experiment. In roots and stems, the highest amount of hyoscyamine and scopolamine are found in vegetative phase with ten rosete leaves and in first stage of flowering phase, respectively. In leaves, the highst quantity of hyoscyamine and scopolamine are in full flowering phase. The flowers have the most amount of scopolamine and hyoscyamine. In fruiting phase, the green and immature seeds in comparison with the mature and brown seeds have more hyoscyamine and scopolamine. In all of these considerated phases, the rate of hyoscyamine production is higher than scopolamine. In vitro culture of Hyoscyamus reticulatus plant was carried out on B5 medium including 2 mg/l NAA and 1 mg/l 6-BAP. Explants were supplied from culturing of root, hypocotyl and leaf of sterile seedlings which were growing in B5 medium free hormone. The effect of different environmental factors on tropane alkaloids production were examined by changing the quantity of sucrose, calcium, nitrate and phosphate in B5 medium.