بومي كاشان MESOBUTHASEUPEUS بررسي فراكسيون هاي زهر كژدم

Surveying the fractions of Mesobuthus eupeus in Kashan

Background: Mesobothus eupeus is the most common type of mesobo thas Belongs to the buthidae family. It is reported all over Iran and is a common cause of biting. Identifying its fractions could be helpful to draw better therapeutic approach, thus the present study was Conducted in Kashan to determine different fractions of mesobu the eupeus. Materials and methods: It was a descriptive study. Having prepared Tricine SDS - PAGE and heated The toyin with a boffer containing mercaptoethanol, 25rnicrograrn, of toxin protein was injected to each plate adjusting the electrophonesis on 80V. Following the electrophoresis, 15hours later, the produced gel was washed and fixed by nethanol. Sigma ultra low MW was used to compare molecular weight of toxin protein Results: Mesobuthus eupeusʹ toxin contains 6 protein bands. The highest one had the molecular weight of 26.96 KD whereas the lowest band had the molecular weight of 1 KD . It has Further proteins with lower molecular weight as compared to Sigma ultra low MW. Conclusion: Since Mesobu thus eupeus Could be found all over Iran, further Studies are Stronaly recommended to determine Better antivenoum.