شاخص هاي استرس اكسيداتيو در بتا- تالاسمي ماژور

PLASMA MEASURES OF OXIDATIVE STRESS IN Beta-THALASSEMIA

Background and Aims: It has been proposed that iron overload in patients with Beta-thalassimia is associated with enhanced free-radical formation and/or defects in antioxidant defense. Iron facilitates the generation of free radical species (i.e. hydroxyl radical) via the Fenton reaction. Hydroxyl radicals are the main cause of tissue damage in biological systems. The aim of the present study was to examine the impact of iron overload in patients with Beta-thalassimia on plasma markers of oxidative stress including vitamin E and malonedialdehyde. Materials and Methods: Patients with major (3-thalassimia and control subjects (age: 7.67± 4.4.2 years and 10.20 Patient with major (3-thalassimia 5.50; n=20 each) were recruited from Imam Hospital, Urmia. Plasma malonedialdehyde levels were analysed spectrophotometrically. Vitamin E concentrations were measured by HPLC coupled to spectrometric detector. Results: Plasma vitamin E levels were lower in patients with Beta-thalassimia than in control subjects (14.0714.25 p.M vs 20.04±5.02 µM; p<0.005). Patients with Beta-thalassimia, on the other hand, had higher malonedialdehyde concentrations than in control subjects (0.34±0.03 µM vs 0.19±0.04 p.M; p<0.005). Both Iron and Ferritin levels were significantly elevated in patients with major P-thalassimia than in control subjects (160.97145.54 µg/L vs 61.16±23.42 µg/L and 387.77-1334.90 gg/L vs 50.29134.15 µg/L, respectively). Discussion: This study has shown that oxidative stress resulting from iron overload in patients with 0-thalassmia is linked with decreased antioxidant capacity and increased lipid peroxidation than in Control subjects.