MOLT-4 تاثير احتمالي سميت سلولي عنصر روي بر سلول هاي رده لنفوييدي مولت -4

EVALUATION OF POSSIBLE CYTOTOXIC EFFECT OF ZINC ON MOLT-4 CELL LINE

Introduction: Zinc has important effects on structural and functional activities of many enzymes, specially regulation of immune system. This study was carried out to examine the in vitro cytotoxic effects of zinc on Molt-4 cell line. Materials & Methods: In this study (by cell culture) the cell line was exposed to different concentration of zinc followed by incubation (37°C, 5% Cot) at various time points (12 to 72 hrs). Viability and proliferation of Molt-4 cells were then evaluated with f iorecent (Ethidium - Bromide and Acridine - Orange) staining and then analysed data by package of SPSS (Dune: and Analysis variants test) were studied and evaluated. Results: The results showed almost different responses to different amount of zinc by the Molt-4 cells less than 100µM at different incubation time points had no effects on cell line when compared to the controls. Higher concentrations of zinc (200-500µM) viability diminshed to 80-85% at 12 hrs and less than 50% at 24 hrs of incubation times. Discussion: We conclude that Zn had dose-dependant cytotoxic effect on Molt-4 cells and probably application for immune-modulation.