اثردرماني جريان مستقيم الكتريسيته بر ليشمانيوز جلدي ناشي از ليشمانيا ماژور در مدل حيواني

THERAPEUTIC EFFECT OF DIRECT CURRENT ELECTRICITY ON CUTANEOUS LEISHMANIASIS DUE TO L.MAJOR IN ANIMAL MODEL

Introduction: Leishmaniasis is a spectrum of disease caused by at least 20 Leishmania sp. in clinical forms of cutaneous, diffused cutaneous, mucocutaneous and visceral. Leishmania major and Leishmania tropica are major causative agents of the cutaneous disease in Iran. At least 80 countries over the world have endemic foci of the disease. For this reason, WHO has emphasized on its prophylaxis and control. The suggested programs that WHO considered, are treatment of patients with the goal of decreasing psycho-social disorders and prevention and control of disease in regions which human is considered as a reservoir. Materials and Methods: In this study, .female BALB/c and NMRI mice in 4-6 week age were distributed in twenty per group in 2 control and case groups. Each mice were challenged in the base of tail by 2 x106 of promastigotes of L.major (MRHO/IR/75/ER) from stationary phase of culture. Various protocols of induction of determined voltages of direct current electricity were used to treat the appeared lesions 2 times per week for 3 weeks. The two control groups received no treatment procedure. This method repeated 3 times because of reproducibility. Results: Variance analyses was used for comparing the results of protocols between the two groups of test and control in both BALB/c and NMRI mice. The results were showed this method can cure all of mice after 3 weeks of induction of electricity. Follow up of the mice resulted in recurrence of the disease in BALB/c mice, not NMRI, next to 3 weeks after finishing treatment. Discussion: Direct Current electricity was evaluated as an effective method of treatment equal to chemical drugs. Electrotherapy in this way, in effectiveness, might eliminate costs and side effects of poisonous antimoniate.