جهت تعيين نقاط عفوني و التهابي در بدن انسان 99M TC-HYNIC-HIGG تهيه كيت

Synthesis, formulation and quality control of 99mTc-HYN1C-hlgG for imaging focal sites of infection and inflamation in human subjects

Imaging of infection and inflammation is an important issue in nuclear medicine as it may have relevant implication for the management of patients with infections or inflammatory diseases. In this regard the synthesis of human polyclonal immunoglobuline G (IgG) radiolabeled with technetium-99m (99ʹTc) by a novel method via a nicotinamide hydrazino derivative, was performed The biological behavior, stability and its high specific activity make this radiopharmaceutical a suitable reagent for radiolabeling of proteins and peptides. In this article the method for synthesis of a sterile and apyrogen 99mTc-HYNIC-hIgG kit is presented The preparation of the kit consists two steps, in the first step 740 MBq pertechnetate was added in Kit No.1, which contains SnCla and tricine After 5 minutes the aliquot in Kit No. I was added to Kit No.2, which contains HYNIC-hIgG 99mTc-HYNIC-hlgG complex is stable in cysteine and serum with a labeling efficiency more than 90% after one hour.