بررسي اختلالات كروموزومي در بيماران مبتلا به سرطان دهان

The assessment of chromosomal abnormalities in patients with oral cancer

Introduction: The aim of this study which was done for the first time in Iran, was the assessment of chromosomal abnormalities in patients with oral cancer. Materials & Methods: For clinical, epidemilogic and probable chromosomal abnormalities evaluation, 45 patients with oral cancer were selected from patients referred to oral disease department of Mashhad dental school and Emam Reza, Ghaem and Omid hospitals. We performed clinical examination and biopsy for confirming diagnosis of oral cancer. After pathology confirmation, two blood samples (containing EDTA and heparin) were prepared from patients who had not received any treatments (radio therapy, chemical therapy or antibiotic therapy) during the previous two weeks. Heparinized blood samples were transported to genetic laboratory for cell culture. Results: Cell culture of 30 patients was done successfully and the other 15 which failed, were excluded from the study. In Karyotype examination, no chromosomal abnormalities were detected in these patients. Conclusion: Minor chromosomal disorders can not he studied through current available procedures in Iran and FISH system which makes further studies available, had not been put into practice in Iran so far. Molecular genetic study and gene isolation can help in detecting gene effect on oral cancer.