تعيين اثر محروميت از آب در دوران جنيني بر هسته دوشكلي جنسي هيپوتالاموس و هورمون هاي جنسي موش نر

Effect of prenatal water deprivation stress on hormone level and sexual dimorphic nucleus hypothalamus in male rats

Introduction: stress in the natural reflection of living creatures against internal and external motivations while stress hormones are secretion through cardiovascular, every production, and security systems. Some stress producing factors are muscular activities, anesthetics, surgery, starvation, water deprivation, etc. Thus, we studied effect of prenatal water deprivation stress on hormone level and sexual dimorphic nucleus in male rats. Materials and methods: In an experimental design, adult female and male Wistar rats (180gr) acquired from Pastoor institute and were coupled. Pregnant rats were divided randomly into control and experimental groups. Both groups were maintained under a controlled temperature (20+2°C) and photoperiod (13:11h, light: dark) conditions. Controlled group had food and water available ad lib, but experimental group had access to food and were deprived of water from the beginning of third week of pregnancy to the end of week. At 42 days at birth, male rats were slight anesthetic in order to prepare the blood and morphometric evaluations. Results: Plasma testosterone average level in experimental group was less than controlled groups (p<0.001), while in the comparison between experimental and controlled groups, progesterone indicates significant increment (p<0.001). There is no statistically significant regarding Estradiol level between both groups. Historical study indicated that the decreased size and number of neurons of MPO and Sch nuclei were significant in experimental compared to controlled ones. Conclusion: Prenatal water deprivation through affecting MPO and Sch nuclei, approximate inhibition of nuclei and decrease in secretion of Testosterone cause the emergence unusual sexual behavior and similar female sexual behaviors in male experimental rats in the postnatal and maturity.