بروسلا آبورتوس در باكتري اشر P93 بيان پروتيين نوتركيب

Expression of recombinant P39 protein from Brucella Abortus in Escherichia coil

Introduction: Brucellosis, caused by Brucella spp., is an important zoonotic disease that causes abortion and infertility in cattle, and undulant fever in human. At present, live attenuated strain 19 of Brucella Abortus is used to immunize domestic animals, but this vaccine has several disadvantages. Therefore, the development of more effective and safer vaccines is necessary for disease control in animals and of more importance in human. So research on development of subunit vaccine seems appropriate, and regarding this, P39 preplasmic protein have shown to be highly immunogenic. Materials and methods: In this experimental study, we amplified P39 gene by Polymerase chain reaction (PCR) method and sub cloned to prokaryotic expression vector pGEX4T1. Escherichia coil 8E21 was transformed with pGEX4T1-P39 and gene expression was induced by IPTG. Then it was purified by GST Sepharose kit. The concentration of P39 was assayed by Bradford method. Results: The recombinant plasmid (pSK-P39) was sequenced. The sequencing result was confirmed by Sanger method and was same as P39 gene. The concentration of purified protein was 200µg/ml. Conclusion: The vectors that contain fusion protein can produce the recombinant P39 protein.