اندازه گيري فعاليت آدنيليل سيكلاز غشاج سلولي در حضور پروتيين كموتاكسيك ماكروفاژ

ADENYLYL CYCLASE ASSAY TO MEASURE MACROPHAGE CHEMOTACTIC PROTEIN-1 RECEPTOR FUNCTION

The purpose of this investigation was to determine the adenylyl cyclase activity for evaluating the role of macrophage chemotactic protein. Adenylyl cyclase is a membrane-bound enzyme that catalyzes the conversion of ATP to CAMP. The inhibition of adenylyl cyclase was carried out by measuring the ability of the macrophage chemotactic protein-1 to inhibit the forskolin-induced enzyme activity. Measurement of adenylyl cyclase activity was performed according to the procedure described by Wiegn. Adenylyl cyclase activity in the present macrophage chemotactic protein-1 was decreased compared to that in controls [2.11+ 0.15(mean + SD.)vs. 6.83 ± 0.45, activity (µmot cAMP/mg protein/min)]. Macrophage chemotactic protein significantly reduced adenylyl cyclase activity by 79.9%.