در داتوراهاي بومي شمال ايران HPLC تعيين مقدار تروپان آلكالوييد تام به روش نيتراسيون و آلكالوييد: هيوسيامين به روش

Quantitative analysis of total alkaloids by titration and Hyoscyamine by HPLC in indigenous Datur species in North of Iran

Background and purpose :.There are ten identified species of Datura in the world. Datura stramonium L. (Torn apple or Jimsonweed) and D.innoxia Mill. (Hindudatura) are endemic species of Datura in Iran, especially in the North (Caspian Sea region). They are distinguished using morphological differences. Form, color and size of leaf, flower, capsule, root and seed are different in D.stranonium and D.innoxia. D.stramonium is more extended than D.innoxia in North of Iran. In fact, Datura stramonium L. is the most widespread species of Datura, in North of Iran. Materials and methods :. Titration and high performance liquid chromatography (HPLC) are outlined in this work for analysis of tropane alkaloids and hyoscyamine in Datura species Results :.Organs (leaf, seed, root). that grow in Mazandaran and Gillan provinces (Caspian sea regions). The percentage of total alkaloids and also the percentage of hyoscyamine of tropanc alkaloids were calculated in leaf, seed and root of the plant. . Conclusion :.maximum tropaneas in D.stramoRivm lea4 (0.40%) and minimum tropane alkaloids was in D. innoxia root(0.18%).