BALB/C بررسي توزيع ماستوسيتهاي رحمي در زمان لانه گزيني پس از سوپراوولاسيون تخمداني در موش آزمايشگاهي نژاد

The Study of Mast Cells Distribution in Mice (balb/c) Uterus After Ovarian Superovulation During Implantation Period

Introduction: Clinical evidence indicates the existence of a narrow window of uterine receptivity which opens during the mid-luteal phase.The polarity of endometrium and formation of pinopodes on the apical surfaces of the endometrial epithelial cells and stromal decidualization occurs at the same time. This study was conducted to find a specific marker for receptivity by evaluating mast cell distribution in the pre-implantation and implantation period after mice ovarian superovulation using human menopausal gonadotropic hormone (hMG) and human chorionic gonadotropic hormones (hCG). Materials & methods: Female balb/c mice (n-28) 6-10 weeks old were housed under standard conditions and divided into controls and superovulated groups. The mice of the control group were rendered pseudo pregnant. Superovulation was done in the experimental group using intraperitoneal injection of 10 i.u hMG, followed by (46 h later) injection of 10 i.u. hCG. On the evening of the second injection the mice were rendered. The mice of each group were sacrificed by cervical dislocation 3.5 and 4.5 days after induction of pseudo pregnancy. Samples were obtained from middle 1/3 part of the uterine horns and fixed and processed for light microscopy and electron microscopic studies. For light microscopy, samples fixed in Bouinʹs solution, processed routinely and tissue sections stained by Toluidine blue reagent. Mast cell counting in controls and experimental groups performed using graticule and analyzed by Student- T tests. For transmission electron microscopy, the samples of the uterine horns were flushed with buffer phosphate sodium cacodylate buffer (pH= 7.2). Samples were fixed first with 2.5% glutaraldehyde in cacodylatebuffer (pH= 7.2) then 1% osmium tetroxide. The specimens dehydrated in gradient concentrations of acetone and then polymerized in resin. Ultra thin sections studied using a transmission electron microscope. Results: Morphometric studies showed significant increase in the number of mastocytes after superovulation in comparison to control groups. In control groups mast cells reside in muscular and adventitial layer but in experimental group there were many mast cells especially in the muscularis layer and lamina propria. Most of the mast cells were in the degranulation stages. Conclusions: Superovulation made significant increase in the number of deregulated mast cells in the preimplantation and implantation periods which may play an inhibitory effect on implantation.