كالوس زايي جنين رسيده بعضي از لاين هاي ذرت تجاري ايران

Callus Induction of Mature Embryos of Some Commercial Maize Inbreds of Iran

Embryogenic callus induction is a critical step for most of gene transformation methods to maize. Unique explant immature embryos for this kind of callus prepration generally limit their production to one or two months a year. Alteration with mature embryo from seed can prolong callus induction during all seasons of the year. In order to reduce this limitation, by using mature seed a factorial experiment including seven important commercial inbreds of Iran and two media conducted via a completely randomize designwas performed. The results showed that callus induction are possible in all lines, if the coleorhizae portion touches medium surface. For most of inbreds mMS2 was better than mN62 but mN62 was better for Mo17 and TVA926. Callus induction speed and durability were depended only on genotype. Lines L105, S61 and TVA926 induced callus in 2 to 3 days but for the other took 8 to 11 days Most durable calf belonged to S61 (328 days) and K722 (291 days) while cali of the other were very in durable, with 68 days for B73 to 109 days for TVA926. Statistical survey of four maintenance media on best line, S61, reveled that N62Ag medium was better than the other maintenance media. Callus of the revealed mentioned line on this medium grew more and finally produced few embryogenic cali but no plant.