بررسي توزيع طبيعي اسيد سياليك در روند اسپرماتوژنز موش

Normal distribution of sialic acid in mouse spermatogenesis

Glycoconjugate have an important role in prolifration , differentition and cell intractions as well as in spermatogenesis and fertilization . Among them , Sialic acid has a very important and unique role . Previous studies have shown this terminal sugar necessity in spermatogenesis , sperm maturation and fertilization .Therefore we decided to determine Sialic acid normal distribution in testicular cells by means of lectin histochemistry and peroxidase labelled Weat germ agglutinin (WGA) lectin , specific for Sialic acid .The pathological states due to different agents could be assessed in comparison with a normal staining pattern . Results show an increase in sialic acid contents from spermatogonia to spermatid formation that followed by sudden reduction in early spermatids . Again in later steps of spermiogenesis to sperm maturation it elevates .There is a moderate and strong reaction in sertoli and leydig cells respectively .These results mean that sialic acid may mask the receptors or cell surface antigens and/or play itself such as a specific receptor in spermatogonial differentiation and meiosis. Elevation of sialic acid in spermiogenesis and mature sperm, rather than a receptor, is related to itʹs role in masking of sperm surface antigens.In leydig cells sialylated glycoconjugates perhaps act such as a testosterone binding structures that transfer it to blood streem and seminiferous tubules.