اثر تزريق درون صفاقي استات روي بر ميزان پرولاكتين سرم در موش هاي صحرايي نر و ماده

The effect of intraperitoneal administration of zinc acetate on serum prolactin in male and female rats

Zinc (Zn) is a trace element which is incorporated into the structure of a variety of cells, hormones and many enzymes. This study was designed to characterize the prolactin status of male and female rats developed during the period of acute and subacute Zn administration. In acute experiments, Zn was administered at doses of 25 and 50 mg/kg; i.p. in 6 rats per group. In subacute experiments, Zn was administered daily for 12 and 16 days at a dose of 15 mg/kg i.p. to 15 rats in each group. Control animals recieved normal saline during the treatment regimen. Both control and Zntreated rats were anesthetized with ether and then sacrificed by decapitation. Blood samples were obtained from each animal after decapitation and prolactin level of 6 rats in each group were measured by R1A. Zinc at doses of 25 or 50 mg/kg caused a significant reduction in serum concentration of prolactin in female rats. However, in male rats, significant decrease in prolactin level were observed only with 50 mg/kg zinc administration. Subacute administration of zinc acetate (15 mg/kg/day for 12 or 16 days) caused a significant reduction in serum prolactin level in both male and female rats compared with the controls. It is concluded that large doses of zinc could result in marked changes in the concentration of prolactin.