نقش اختصاصي ان - استيل گالاكتوز آمين در تبديل سلول هاي متراكم مزانشيم جوانه اندام به غضروف در موش

Role of N-aceetylgalactoseamine in transformation of condense mesenchyme into cartilage-

Extracellular matrix and cell-coat complex carbohydrates play a major role in critical cellular functions. The purpose of the present study was to investigate the role of certain glycoconjugates during transition of the limb bud core mesenchymal cells into cartilage. Using histochemical procedures, embryonic mouse limb buds from days 10 to 16 of development were processed and examined with HRP-lectins including DBA, VVA, VVA/B4 and SBA, to localize special glycoconjugates containing terminal N-acetylgalactosamine. The results showed striking reaction of only VVA and VVAB4 with the core limb condensed mesenchyme. The timing and distribution of staining with VVA and VVA/B4 suggest that specific terminal sugar may play a role in transition of mesenchyme into core condense cells and finally into pre-cartilage stage and subsequent formation of the cartilage.