مقايسه تهيه نمونه بافت هاي نرم به روشهاي سريع و معمولي جهت استفاده در ميكروسكوپ الكتروني از نوع ترانسميشن

A comparison between the normal and rapid methods in processing of soft tissues used in transmision electron microscopy

The requirement of long time and the usage of highly volatile carcinogen solvent, i. e. propylene oxide in tissue processing have created limitations in the ultimate usage of Transmission Electron Microscopy (TEM) as a valuable tool in biological studies and diagnostic procedures. In this study, as the soft tissues the liver, skin, brain and intestine of a Albino Swiss Mice, Charles Revere Strain, were processed by applying a normal method requiring 74 hours and a rapid method which proposed to be 6 hours. In rapid method, the size of the specimens were reduced to 0.5 cubic millimeter, collidine-HC1 was used to facilitate the penetration of osmium tetroxide in the tissues and the tissues were cured in 85 °C . In each method, the use of acetone as a low toxic solvent was compared with propylene oxide and their influences in the quality of the cellular structures and the parametric changes of the organelles of different target cells were studied. By comparing the results, it was shown that the quality of the studied tissues were preserved and meaningful (p<0.05) changes in the sizes of the organelles of the studied cells were observed. The results revealed that by the use of acetone instead of propylene oxide, the time of processing will be reduced and the whole procedure will be more convenient. Therefore, comparing with the long method by the use of propylene oxide, rapid method by the use of acetone could be more convenient in soft tissue processing for TEM, using the procedures described in this study.