در نمونه هاي باختي لنفوم هاي اوليه گوارشي تشخيص داده شده در 22 اخير ... HTLV-1 بررسي ژنوم

Detection of HTLV-1 genome in primary gastrointestinal lymphomas:A study on 22 years (1981- 2003) pathology archive biopsy specimens in Imam Reza Hospital, Mashhad, Iran

Non-Hodgkinʹs lymphoma (NHL) with primary gastrointestinal localization (G1-NHL) represents only I -4% of the malignant tumors in the gastrointestinal tract. However, the gastrointestinal tract is the most common extra-nodal site of NHL. The etiology of GI-NHL remains unknown, however, recent studies have shown that infection with viruses like HTLV-l in the gastrointestinal tract may increase the risk of T cell NHL. The present study was designed to investigate the presence of HMV-1 genome using PCR on paraffin embedded tissue of primary G1-NHL specimens diagnosed during the period from 1981 to 2003 in the Department of Pathology, Imam Reza Hospital, Mashhad, Iran. The total number of biopsies was 103, 573; and 65 cases with primary GI-NHL were found. All these 65 specimens were stained with immunohistochemical markers such as LCA, CD20 and CD3. The presence of HMV-I genome was detected in 48 selected patients using tax, LTR, pot and enl‘(GD21) primers. Our results show that male to female distribution was 1.5:1. Most of the patients were in their second or third decades of life. Eight patients (12.3%) had primary lymphoma in the stomach, 52 (80%) in the small intestine, two (3%) in the large intestine, and three (4.7%) patients had multifocal involvements (ileocecal). According to the Revised European-American Lymphoma (REAL) classification. IPS1D lymphoma was the most frequent histologic subtype, comprising 40% of the cases. Sixty-four eases were classified as 13 cell lymphomas, and one as T-cell lymphoma. Out of 48 specimens which were amplified using PcR, two (4.2%) were positive for HTLV-1. The first case was a 55-year old male with diffuse pleomorphic T cell lymphoma who had a lesion in stomach; and the second ease was a 15-month-old female with IPSID Iymphoma who had involvement at duodenum.