بررسي اثرات ضدتهوعي فراكسيون هاي اندام هاي هوايي گياه پونه در جوجه

Antiemetic effect of Mentha longifolia aerial parts extracts and fractions in young chickens

The antiemetic effect of Mentha longifolia extracts and fractions was studied in young chickens in order to provide a basis for the traditional use of the plant. Emesis was induced with copper sulfate (60 mg/kg, orally) and ipecac suspension (600 mg/kg, orally). The LD50 values of aqueous and methanolic extracts were 1561.7 mg/kg and 2161.6 mg/kg, respectively. The Maximum non-fatal doses of aqueous and methanolic extracts were 100 mg/kg and 800 mg/kg, respectively. The methanolic extract showed more efficacy, therefore the chloroformic and aqueous-methanolie fractions were prepared from it. Because of the better appearance and solubility of aqueous-methanolic fraction, other fractions were prepared from it by the method of MPLC. Among these, fractions C and F showed the most efficacy. In all groups, the positive control (metoclopramide) showed the most efficacy. In each step, more efficacy with lower dose showed that the extraction procedure was effective. Finally, fraction F showed more efficacy than fraction C and both of these fractions demonstrated more efficacy than the aqueous-methanolic fraction. According to these results, the aqueous and methanolic extracts as well as the fractions of Mentha longifolia showed antiemetic effects in young chickens with central and peripheral mechanisms.