در مقابل صدمات كروموزومي وارده بر سلولهاي مغز استخوان موش ... E و A استفاده از آزمون ميكرونوكليوس در بررسي اثر حفاظتي ويتامينهاي

Analysis of protection effect of vitamins A, E against Gamma induced chromosomal abnormalities post and past-irradiation on mouse bone marrow cells using micronucleus assay

The radioprotectory effect of vitamins A and E post and past Gamma-irradiation on mouse bone marrow cells using micronucleus assay was analyzed. Micronucleus assay is a very fast and effective method in evaluating structural as well as numerical chromosomal abnormalities. Search for finding an effective way of protection against harmful effects of induced or spontaneous irradiation is actively progressing. Effects of antioxidants such as vitamin A and E in in vivo have studied extensively. In this study effect of different doses of vitamin A and E post and past Gamma-irradiation of 2 Gy were analyzed. Results showed that both vitamins were able to reduce frequency of micronuclei which were representing chromosomal damages. Of all doses used ( 0.6mg/kg, 1.2mg/kg and 2.4mg/kg for vitamin A and 50mg/kg, 100mg/kg and 200mg/kg for vitamin E), 2.4mglkg body weight of vitamin A and 200mg/kg body weight of vitamin E had the best effect on reducing induced-damages. Combination of two vitamins in these doses post and past irradiation revealed that vitamin E was the most effective post irradiation whereas vitamin A was the most effective past irradiation in reducing the frequency of micronucleus. According to our knowledge this is the first time that the anticlastogenic effect of vitamin A and E post and past irradiation in in vivo has been studied at the same time using micronucleus assay.